ArticleMolecular neurodegeneration2011
An organotypic slice culture model of chronic white matter injury with maturation arrest of oligodendrocyte progenitors.
Article in Molecular neurodegeneration, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 34 citations in OpenAlex.
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- Zika virus induced microcephaly and aberrant hematopoietic cell differentiation modeled in novel neonatal humanized mice.Frontiers in immunology · 2023Article
- Tenascins Interfere With Remyelination in an Ex Vivo Cerebellar Explant Model of Demyelination.Frontiers in cell and developmental biology · 2022Article
- Early postnatal irradiation-induced age-dependent changes in adult mouse brain: MRI based characterization.BMC neuroscience · 2021Article
- Anti-Inflammatory Therapies for Treatment of Inflammation-Related Preterm Brain Injury.International journal of molecular sciences · 2021Review
- Dysregulation of Hyaluronan Homeostasis During White Matter Injury.Neurochemical research · 2020Review
- A modified flavonoid accelerates oligodendrocyte maturation and functional remyelination.Glia · 2020Article
- A TLR/AKT/FoxO3 immune tolerance-like pathway disrupts the repair capacity of oligodendrocyte progenitors.The Journal of clinical investigation · 2018Article
- White matter damage after traumatic brain injury: A role for damage associated molecular patterns.Biochimica et biophysica acta. Molecular basis of disease · 2017Review
- The axon-glia unit in white matter stroke: mechanisms of damage and recovery.Brain research · 2015Review
- In vitro myelin formation using embryonic stem cells.Development (Cambridge, England) · 2015Article
- Hyaluronan Synthesis, Catabolism, and Signaling in Neurodegenerative Diseases.International journal of cell biology · 2015Review
- White matter injury induced by perinatal exposure to glutaric acid.Neurotoxicity research · 2014Article
- Rat cerebellar slice cultures exposed to bilirubin evidence reactive gliosis, excitotoxicity and impaired myelinogenesis that is prevented by AMPA and TNF-α inhibitors.Molecular neurobiology · 2014Article
- Modeling the encephalopathy of prematurity in animals: the important role of translational research.Neurology research international · 2012Article
- Brain development in rodents and humans: Identifying benchmarks of maturation and vulnerability to injury across species.Progress in neurobiologyReview
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
backgroundCNS myelination disturbances commonly occur in chronic white matter lesions in neurodevelopmental and adult neurological disorders. Recent studies support that myelination failure can involve a disrupted cellular repair mechanism where oligodendrocyte (OL) progenitor cells (OPCs) proliferate in lesions with diffuse astrogliosis, but fail to fully differentiate to mature myelinating OLs. There are no in vitro models that reproduce these features of myelination failure.
resultsForebrain coronal slices from postnatal day (P) 0.5/1 rat pups were cultured for 1, 5, or 9 days in vitro (DIV). Slices rapidly exhibited diffuse astrogliosis and accumulation of the extracellular matrix glycosaminoglycan hyaluronan (HA), an inhibitor of OPC differentiation and re-myelination. At 1 DIV ~1.5% of Olig2+ OLs displayed caspase-3 activation, which increased to ~11.5% by 9 DIV. At 1 DIV the density of PDGFRα+ and PDGFRα+/Ki67+ OPCs were significantly elevated compared to 0 DIV (P < 0.01). Despite this proliferative response, at 9 DIV ~60% of white matter OLs were late progenitors (preOLs), compared to ~7% in the postnatal day 10 rat (P < 0.0001), consistent with preOL maturation arrest. Addition of HA to slices significantly decreased the density of MBP+ OLs at 9 DIV compared to controls (217 ± 16 vs. 328 ± 17 cells/mm2, respectively; P = 0.0003), supporting an inhibitory role of HA in OL lineage progression in chronic lesions.
conclusionsDiffuse white matter astrogliosis and early OPC proliferation with impaired OL maturation were reproduced in this model of myelination failure. This system may be used to define mechanisms of OPC maturation arrest and myelination failure related to astrogliosis and HA accumulation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.