ArticleMolecular and cellular biology1990
Transcription factor Sp1 is important for retinoic acid-induced expression of the tissue plasminogen activator gene during F9 teratocarcinoma cell differentiation.
Article in Molecular and cellular biology, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 64 citations in OpenAlex.
- MiR-340 Regulates Fibrinolysis and Axon Regrowth Following Sciatic Nerve Injury.Molecular neurobiology · 2017Article
- Cell-type specific DNA-protein interactions at the tissue-type plasminogen activator promoter in human endothelial and HeLa cells in vivo and in vitro.Nucleic acids research · 1997Article
- Sp1 binds two sites in the CD11c promoter in vivo specifically in myeloid cells and cooperates with AP1 to activate transcription.Molecular and cellular biology · 1996Article
- G10BP, an E1A-inducible negative regulator of Sp1, represses transcription of the rat fibronectin gene.Molecular and cellular biology · 1995Article
- Aryl hydrocarbon-induced interactions at multiple DNA elements of diverse sequence--a multicomponent mechanism for activation of cytochrome P4501A1 (CYP1A1) gene transcription.Nucleic acids research · 1994Article
- Retinoic acid induction of major histocompatibility complex class I genes in NTera-2 embryonal carcinoma cells involves induction of NF-kappa B (p50-p65) and retinoic acid receptor beta-retinoid X receptor beta heterodimers.Molecular and cellular biology · 1993Article
- Genomic structure and chromosomal localization of the human deoxycytidine kinase gene.Proceedings of the National Academy of Sciences of the United States of America · 1993Article
- Transcriptional regulation of the rat tissue type plasminogen activator gene: localization of DNA elements and nuclear factors mediating constitutive and cyclic AMP-induced expression.Molecular and cellular biology · 1993Article
- Induction of E1A-responsive negative factors for transcription of the fibronectin gene in adenovirus E1-transformed rat cells.Journal of virology · 1992Article
- Transcriptional regulation of transferrin and albumin genes by retinoic acid in human hepatoma cell line Hep3B.The Biochemical journal · 1992Article
- Thrombomodulin gene regulation by cAMP and retinoic acid in F9 embryonal carcinoma cells.Proceedings of the National Academy of Sciences of the United States of America · 1992Article
- Cyclic AMP response element-binding protein and the catalytic subunit of protein kinase A are present in F9 embryonal carcinoma cells but are unable to activate the somatostatin promoter.Molecular and cellular biology · 1992Article
- Regulation of E2F/cyclin A-containing complex upon retinoic acid-induced differentiation of teratocarcinoma cells.Gene expression · 1992Article
- In vitro analysis of the tissue plasminogen activator promoter reveals a GC box-binding activity present in murine brain but undetectable in kidney and liver.Molecular and cellular biology · 1991Article
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4 authors.
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No grant is acknowledged in the PubMed record.
Abstract
The induced differentiation of F9 cells by retinoic acid (RA) and cyclic AMP (cAMP) activated transcription of the tissue plasminogen activator (t-PA) gene. This differentiation-responsive regulation of the t-PA promoter was also observed in transient assays. Multiple sequence elements within 243 bp of t-PA DNA contributed to the high level of transcription in retinoic acid- and cyclic AMP-differentiated cells. To investigate the factors involved in controlling t-PA transcription upon differentiation, we used F9 cell extracts to examine proteins that bind two proximal promoter elements. These elements (boxes 4 and 5) are homologous to GC boxes that are known binding sites for transcription factor Sp1. Mobility shift assays in the presence and absence of anti-Sp1 antibodies demonstrated that the proteins which bound to this region were immunologically related to human Sp1. The proteins also had a DNA-binding specificity similar to that of a truncated form of Sp1. Mutations of the GC motif within boxes 4 and 5 that interfered with Sp1 binding reduced in parallel the binding of the F9 cellular factors and lowered transcription in vitro as well as in vivo. Although this proximal region of the t-PA promoter was active in vivo only in differentiated cells, the Sp1-like binding proteins were present in equal concentrations and had similar properties in extracts of both stem and differentiated cells. These data suggest that other cellular elements participate with this Sp1-like factor in controlling differentiation-specific expression.
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