ArticleNucleic acids research2011
Modulation of the stability of the Salmonella fourU-type RNA thermometer.
Article in Nucleic acids research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed.
- RNA thermometers as temperature-dependent tools for enhanced recombinant protein production in precision fermentation.Microbial cell factories · 2026Article
- Localized ribosome access and distal tuning via thebioRxiv : the preprint server for biology · 2026Article
- SUPER: Upcycling Genetic Parts for Precise Gene Expression Control, Leakage Minimization, and Genetic Circuit Stability.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Temperature-Dependent Recombinase-Based Genetic Circuits.International journal of molecular sciences · 2025Article
- Advances in solid-state NMR methods for studying RNA structures and dynamics.Magnetic resonance letters · 2025Review
- The oxidative stress response, in particular the katY gene, is temperature-regulated in Yersinia pseudotuberculosis.PLoS genetics · 2023Article
- Article
- Temperature Matters: Bacterial Response to Temperature Change.Journal of microbiology (Seoul, Korea) · 2023Review
- Dynamics of base pairs with low stability in RNA by solid-state nuclear magnetic resonance exchange spectroscopy.iScience · 2022Article
- The effect of mutation in the stem of the MicroROSE thermometer on its thermosensing ability: insights from molecular dynamics simulation studies.RSC advances · 2022Article
- Article
- Exploiting RNA thermometer-driven molecular bioprocess control as a concept for heterologous rhamnolipid production.Scientific reports · 2021Article
- Probing the physiological roles of the extracellular loops of chitoporin from Vibrio campbellii.Biophysical journal · 2021Article
- RNA thermosensors facilitate Streptococcus pneumoniae and Haemophilus influenzae immune evasion.PLoS pathogens · 2021Article
- An RNA thermometer dictates production of a secreted bacterial toxin.PLoS pathogens · 2020Article
- Temperature Control ofJournal of bacteriology · 2019Article
- Bacterial riboswitches and RNA thermometers: Nature and contributions to pathogenesis.Non-coding RNA research · 2018Review
- RNA Thermometers in Bacterial Pathogens.Microbiology spectrum · 2018Review
- Characterizing the Structure-Function Relationship of a Naturally Occurring RNA Thermometer.Biochemistry · 2017Article
- The Potential Link between Thermal Resistance and Virulence inFrontiers in veterinary science · 2017Review
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA thermometers are translational control elements that regulate the expression of bacterial heat shock and virulence genes. They fold into complex secondary structures that block translation at low temperatures. A temperature increase releases the ribosome binding site and thus permits translation initiation. In fourU-type RNA thermometers, the AGGA sequence of the SD region is paired with four consecutive uridines. We investigated the melting points of the wild-type and mutant sequences. It was decreased by 5°C when a stabilizing GC basepair was exchanged by an AU pair or increased by 11°C when an internal AG mismatch was converted to a GC pair, respectively. Stabilized or destabilized RNA structures are directly correlated with decreased or increased in vivo gene expression, respectively. Mg(2+) also affected the melting point of the fourU thermometer. Variations of the Mg(2+) concentration in the physiological range between 1 and 2 mM translated into a 2.8°C shift of the melting point. Thus, Mg(2+) binding to the hairpin RNA is regulatory relevant. Applying three different NMR techniques, two Mg(2+) binding sites were found in the hairpin structure. One of these binding sites could be identified as outer sphere binding site that is located within the fourU motif. Binding of the two Mg(2+) ions exhibits a positive cooperativity with a Hill coefficient of 1.47. Free energy values ΔG for Mg(2+) binding determined by NMR are in agreement with data determined from CD measurements.
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