ArticleMolecular plant pathology2011
Altered trichothecene biosynthesis in TRI6-silenced transformants of Fusarium culmorum influences the severity of crown and foot rot on durum wheat seedlings.
Article in Molecular plant pathology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 61 citations in OpenAlex.
- Article
- Harnessing RNA interference for the control of Fusarium species: A critical review.Molecular plant pathology · 2024Review
- Efficacy ofFrontiers in microbiology · 2024Article
- Containment ofJournal of fungi (Basel, Switzerland) · 2023Article
- Effect of Compactin on the Mycotoxin Production and Expression of Related Biosynthetic and Regulatory Genes in ToxigenicMicroorganisms · 2022Article
- Review
- Genome-wide association study for deoxynivalenol production and aggressiveness in wheat and rye head blight by resequencing 92 isolates of Fusarium culmorum.BMC genomics · 2021Article
- Transcription factor control of virulence in phytopathogenic fungi.Molecular plant pathology · 2021Review
- Trichothecenes in Cereal Grains - An Update.Toxins · 2019Review
- CRISPR-Cas for Fungal Genome Editing: A New Tool for the Management of Plant Diseases.Frontiers in plant science · 2019Article
- Equipping Durum Wheat-Frontiers in plant science · 2019Article
- Investigation of Camphor Effects onPathogens (Basel, Switzerland) · 2018Article
- The cereal pathogen Fusarium pseudograminearum produces a new class of active cytokinins during infection.Molecular plant pathology · 2018Article
- FcRav2, a gene with a ROGDI domain involved in Fusarium head blight and crown rot on durum wheat caused by Fusarium culmorum.Molecular plant pathology · 2018Article
- Transcriptomics of cereal-Fusarium graminearum interactions: what we have learned so far.Molecular plant pathology · 2018Review
- Article
- Candidate gene based association mapping in Fusarium culmorum for field quantitative pathogenicity and mycotoxin production in wheat.BMC genetics · 2017Article
- Annotation of Fusarium graminearum (PH-1) Version 5.0.Genome announcements · 2017Article
- First Draft Genome Sequence of a UK Strain (UK99) of Fusarium culmorum.Genome announcements · 2016Article
- Article
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An RNA silencing construct was used to alter mycotoxin production in the plant pathogenic fungus Fusarium culmorum, the incitant of crown and foot rot on wheat. The transformation of a wild-type strain and its nitrate reductase-deficient mutant with inverted repeat transgenes (IRTs) containing sequences corresponding to the trichothecene regulatory gene TRI6 was achieved using hygromycin B resistance as a selectable marker. Southern analysis revealed a variety of integration patterns of the TRI6 IRT. One transformant underwent homologous recombination with deletion of the endogenous TRI6 gene, whereas, in another transformant, the TRI6 IRT was not integrated into the genome. The TRI6 IRT did not alter the physiological characteristics, such as spore production, pigmentation or growth rate, on solid media. In most transformants, a high TRI6 amplification signal was detected by quantitative reverse transcription-polymerase chain reaction, corresponding to a TRI6-hybridizing smear of degraded fragments by Northern analysis, whereas TRI5 expression decreased compared with the respective nontransformed strain. Four transformants showed increased TRI5 expression, which was correlated with a dramatic (up to 28-fold) augmentation of deoxynivalenol production. Pathogenicity assays on durum wheat seedlings confirmed that impairment of deoxynivalenol production in the TRI6 IRT transformants correlated with a loss of virulence, with decreased disease indices ranging from 40% to 80% in nine silenced strains, whereas the overproducing transformants displayed higher virulence compared with the wild-type.
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