ArticleJournal of proteome research2011
Rapid verification of candidate serological biomarkers using gel-based, label-free multiple reaction monitoring.
Article in Journal of proteome research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed.
- A label-free selected reaction monitoring workflow identifies a subset of pregnancy specific glycoproteins as potential predictive markers of early-onset pre-eclampsia.Molecular & cellular proteomics : MCP · 2013Trial
- Protein isoform-specific validation defines multiple chloride intracellular channel and tropomyosin isoforms as serological biomarkers of ovarian cancer.Journal of proteomics · 2013Trial
- Screening of immune biomarkers in different breeds of chickens infected with J subgroup of avian leukemia virus by proteomic.Virulence · 2020Article
- Proteomic Analysis of the Early Development of theMolecules (Basel, Switzerland) · 2020Article
- Article
- LC-SRM-Based Targeted Quantification of Urinary Protein Biomarkers.Methods in molecular biology (Clifton, N.J.) · 2018Article
- Multiplexed Liquid Chromatography-Multiple Reaction Monitoring Mass Spectrometry Quantification of Cancer Signaling Proteins.Methods in molecular biology (Clifton, N.J.) · 2017Article
- Review
- Blood-Based Proteomic Biomarkers of Alzheimer's Disease Pathology.Frontiers in neurology · 2015Review
- Identification and Quantitation of Coding Variants and Isoforms of Pulmonary Surfactant Protein A.Journal of proteome research · 2014Article
- Targeted peptide measurements in biology and medicine: best practices for mass spectrometry-based assay development using a fit-for-purpose approach.Molecular & cellular proteomics : MCP · 2014Article
- Comparative secretome analysis of epithelial and mesenchymal subpopulations of head and neck squamous cell carcinoma identifies S100A4 as a potential therapeutic target.Molecular & cellular proteomics : MCP · 2013Article
- Whole gel processing procedure for GeLC-MS/MS based proteomics.Proteome science · 2013Article
- Systematic comparison of fractionation methods for in-depth analysis of plasma proteomes.Journal of proteome research · 2012Article
- Advancing the sensitivity of selected reaction monitoring-based targeted quantitative proteomics.Proteomics · 2012Review
- A xenograft mouse model coupled with in-depth plasma proteome analysis facilitates identification of novel serum biomarkers for human ovarian cancer.Journal of proteome research · 2012Article
- Deciphering mitochondrial metabolic vulnerabilities in ovarian clear cell carcinoma with mass spectrometry-based clinical proteomics.Expert review of proteomicsReview
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Stable isotope dilution-multiple reaction monitoring-mass spectrometry (SID-MRM-MS) has emerged as a promising platform for verification of serological candidate biomarkers. However, cost and time needed to synthesize and evaluate stable isotope peptides, optimize spike-in assays, and generate standard curves quickly becomes unattractive when testing many candidate biomarkers. In this study, we demonstrate that label-free multiplexed MRM-MS coupled with major protein depletion and 1D gel separation is a time-efficient, cost-effective initial biomarker verification strategy requiring less than 100 μL of serum. Furthermore, SDS gel fractionation can resolve different molecular weight forms of targeted proteins with potential diagnostic value. Because fractionation is at the protein level, consistency of peptide quantitation profiles across fractions permits rapid detection of quantitation problems for specific peptides from a given protein. Despite the lack of internal standards, the entire workflow can be highly reproducible, and long-term reproducibility of relative protein abundance can be obtained using different mass spectrometers and LC methods with external reference standards. Quantitation down to ~200 pg/mL could be achieved using this workflow. Hence, the label-free GeLC-MRM workflow enables rapid, sensitive, and economical initial screening of large numbers of candidate biomarkers prior to setting up SID-MRM assays or immunoassays for the most promising candidate biomarkers.
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Registered trials
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