ArticlePlant molecular biology2011
Heterotrimeric Gα subunit from wheat (Triticum aestivum), GA3, interacts with the calcium-binding protein, Clo3, and the phosphoinositide-specific phospholipase C, PI-PLC1.
Article in Plant molecular biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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30 citing papers in PubMed, 54 citations in OpenAlex.
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- Origin, evolution and diversification of plant caleosins.BMC plant biology · 2025Article
- Plant PI-PLC signaling in stress and development.Plant physiology · 2025Review
- Advances and opportunities in unraveling cold-tolerance mechanisms in the world's primary staple food crops.The plant genome · 2024Review
- Caleosin/peroxygenases: multifunctional proteins in plants.Annals of botany · 2023Review
- Recent Advances in Life History Transition with Nematode-Trapping FungusPathogens (Basel, Switzerland) · 2023Review
- A computational study on the structure-function relationships of plant caleosins.Scientific reports · 2023Article
- MiningFrontiers in plant science · 2023Article
- Regulatory Mechanism of Trap Formation in the Nematode-Trapping Fungi.Journal of fungi (Basel, Switzerland) · 2022Review
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- The functions of phospholipases and their hydrolysis products in plant growth, development and stress responses.Progress in lipid research · 2022Review
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- Aegilops tauschii genome assembly Aet v5.0 features greater sequence contiguity and improved annotation.G3 (Bethesda, Md.) · 2021Article
- Emerging role of phospholipase C mediated lipid signaling in abiotic stress tolerance and development in plants.Plant cell reports · 2021Review
- The stress induced caleosin, RD20/CLO3, acts as a negative regulator of GPA1 in Arabidopsis.Plant molecular biology · 2021Article
- Phosphoinositide-specific phospholipase C gene involved in heat and drought tolerance in wheat (Triticum aestivum L.).Genes & genomics · 2021Article
- Evolution and Stress Responses ofFrontiers in plant science · 2021Article
- The CsGPA1-CsAQPs module is essential for salt tolerance of cucumber seedlings.Plant cell reports · 2020Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The canonical Gα subunit of the heterotrimeric G protein complex from wheat (Triticum aestivum), GA3, and the calcium-binding protein, Clo3, were revealed to interact both in vivo and in vitro and Clo3 was shown to enhance the GTPase activity of GA3. Clo3 is a member of the caleosin gene family in wheat with a single EF-hand domain and is induced during cold acclimation. Bimolecular Fluorescent Complementation (BiFC) was used to localize the interaction between Clo3 and GA3 to the plasma membrane (PM). Even though heterotrimeric G-protein signaling and Ca²⁺ signaling have both been shown to play a role in the response to environmental stresses in plants, little is known about the interaction between calcium-binding proteins and Gα. The GAP activity of Clo3 towards GA3 suggests it may play a role in the inactivation of GA3 as part of the stress response in plants. GA3 was also shown to interact with the phosphoinositide-specific phospholipase C, PI-PLC1, not only in the PM but also in the endoplasmic reticulum (ER). Surprisingly, Clo3 was also shown to interact with PI-PLC1 in the PM and ER. In vitro analysis of the protein-protein interaction showed that the interaction of Clo3 with GA3 and PI-PLC1 is enhanced by high Ca²⁺ levels. Three-way affinity characterizations with GA3, Clo3 and PI-PLC1 showed the interaction with Clo3 to be competitive, which suggests that Clo3 may play a role in the Ca²⁺-triggered feedback regulation of both GA3 and PI-PLC1. This hypothesis was further supported by the demonstration that Clo3 has GAP activity with GA3.
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