ArticleAsian journal of andrology2011
The ability of sperm selection techniques to remove single- or double-strand DNA damage.
Article in Asian journal of andrology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed.
- Density gradient centrifugation versus swim-up for selecting spermatozoa with improved redox activity, calcium status, and DNA integrity in an established rat model of varicocele-induced infertility.Frontiers in cell and developmental biology · 2026Article
- Sperm selection for assisted reproductive technology: do we have a winner?Journal of assisted reproduction and genetics · 2025Review
- Sperm Separation and Selection Techniques to Mitigate Sperm DNA Damage.Life (Basel, Switzerland) · 2025Review
- Advanced Sperm Selection Techniques for Assisted Reproduction.Journal of personalized medicine · 2024Review
- Comparison between density gradient centrifugation method, an extended version of the horizontal swim up method and the combination of both for sperm selection.Obstetrics & gynecology science · 2023Article
- Double strand DNA breaks in sperm: the bad guy in the crowd.Journal of assisted reproduction and genetics · 2023Article
- Effects of sperm preparation techniques on sperm survivability and DNA fragmentation.The Journal of international medical research · 2022Article
- Single-cell sperm transcriptomes and variants from fathers of children with and without autism spectrum disorder.NPJ genomic medicine · 2020Article
- A treatment approach for couples with disrupted sperm DNA integrity and recurrent ART failure.Journal of assisted reproduction and genetics · 2019Article
- Article
- Evaluation of normal morphology, DNA fragmentation, and hyaluronic acid binding ability of human spermatozoa after using four different commercial media for density gradient centrifugation.Clinical and experimental reproductive medicine · 2019Article
- Sperm DNA fragmentation and sex chromosome aneuploidy after swim-up versus density gradient centrifugation.Clinical and experimental reproductive medicine · 2017Article
- ICSI significantly improved the pregnancy rate of patients with a high sperm DNA fragmentation index.Clinical and experimental reproductive medicine · 2017Article
- Efficient isolation of sperm with high DNA integrity and stable chromatin packaging by a combination of density-gradient centrifugation and magnetic-activated cell sorting.Clinical and experimental reproductive medicine · 2016Article
- An Update on Oxidative Damage to Spermatozoa and Oocytes.BioMed research international · 2016Review
- Sperm quality after density gradient centrifugation with three commercially available media: a controlled trial.Reproductive biology and endocrinology : RB&E · 2014Article
- Interpreting sperm DNA damage in a diverse range of mammalian sperm by means of the two-tailed comet assay.Frontiers in genetics · 2014Review
- Can DNA fragmentation of neat or swim-up spermatozoa be used to predict pregnancy following ICSI of fertile oocyte donors?Asian journal of andrology · 2013Article
- Intracytoplasmic morphologically selected sperm injection outcomes: the role of sperm preparation techniques.Journal of assisted reproduction and genetics · 2013Article
- SwimCount Harvester™ versus Density Gradient Centrifugation: A Pilot Study exploring Sperm DNA Fragmentation and Motile Sperm Recovery.International braz j urol : official journal of the Brazilian Society of UrologyArticle
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A wide variety of techniques for the preparation of sperm are currently available, of which the most commonly employed are density-gradient centrifugation (DGC) and swim-up (SUP). To date, these methods appear to be effective in selecting functional sperm for assisted reproduction techniques (ART), but they may have negative effects on sperm DNA. In this study, the ability of these semen processing techniques to eliminate spermatozoa containing single- and double-strand DNA damage was assessed by the two-tailed comet assay and the sperm chromatin dispersion test in 157 semen samples from patients seeking assisted reproduction treatment. Our results indicated that SUP and DGC are equally efficient in eliminating spermatozoa containing double-strand DNA damage and sperm with highly damaged (degraded) DNA, as characterized by the presence of both single- and double-strand DNA breaks. However, DGC is more efficient than SUP in selecting spermatozoa that are free from single-strand DNA damage. Future studies should characterise the importance of the various types of DNA damage and examine the sperm processing protocols used in each laboratory to determine their ability to eliminate DNA damage and hence, prevent the potential transmission of genetic mutations via ART.
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Registered trials
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