ArticleJournal of bacteriology2011
An ABC-type cobalt transport system is essential for growth of Sinorhizobium meliloti at trace metal concentrations.
Article in Journal of bacteriology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 51 citations in OpenAlex.
- A minimized symbiotic gene set from the 1.68 Mb pSymB chromid of Sinorhizobium meliloti reveals auxiliary symbiotic loci.BMC biology · 2025Article
- Skin-associatedmSphere · 2025Article
- Skin-associatedbioRxiv : the preprint server for biology · 2024Article
- Metal nutrition and transport in the process of symbiotic nitrogen fixation.Plant communications · 2024Review
- Evolutionary engineering and molecular characterization of cobalt-resistantFrontiers in microbiology · 2024Article
- Functional Characterization of the CoApplied and environmental microbiology · 2023Article
- Metals to combat antimicrobial resistance.Nature reviews. Chemistry · 2023Review
- Exploring the onset of BEnvironmental microbiology · 2022Article
- The Biochemical Landscape of Riboswitch Ligands.Biochemistry · 2022Article
- The repertoire of ABC proteins inComputational and structural biotechnology journal · 2021Article
- The requirement for cobalt in vitamin BBiochimica et biophysica acta. Molecular cell research · 2021Review
- Metabolic engineering of Escherichia coli for de novo biosynthesis of vitamin BNature communications · 2018Article
- Inter-replicon Gene Flow Contributes to Transcriptional Integration in theG3 (Bethesda, Md.) · 2018Article
- Novel Genes and Regulators That Influence Production of Cell Surface Exopolysaccharides in Sinorhizobium meliloti.Journal of bacteriology · 2018Article
- An account of evolutionary specialization: the AbcR small RNAs in the Rhizobiales.Molecular microbiology · 2018Review
- Loss of malic enzymes leads to metabolic imbalance and altered levels of trehalose and putrescine in the bacterium Sinorhizobium meliloti.BMC microbiology · 2016Article
- Metabolic modelling reveals the specialization of secondary replicons for niche adaptation in Sinorhizobium meliloti.Nature communications · 2016Article
- Genetic redundancy is prevalent within the 6.7 Mb Sinorhizobium meliloti genome.Molecular genetics and genomics : MGG · 2015Article
- Rho and RNase play a central role in FMN riboswitch regulation in Corynebacterium glutamicum.Nucleic acids research · 2015Article
- Examination of prokaryotic multipartite genome evolution through experimental genome reduction.PLoS genetics · 2014Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We report expression and mutant phenotypes for a gene cluster in Sinorhizobium meliloti, designated cbtJKL, that has been shown to encode an ABC-type cobalt transport system. Transcription of cbtJKL initiated 384 nucleotides upstream from the cbtJ translation start codon, and the resulting 5' region contained a putative B(12)riboswitch. Expression of the cbtJKL genes appeared to be controlled by (cobalt-loaded) cobalamin interacting at the B(12)riboswitch, since (i) a putative B(12)riboswitch was located within this large upstream region, (ii) cbtJ transcription was repressed upon addition of cobalt or vitamin B(12), and (iii) deletions in the B(12)riboswitch resulted in constitutive cbtJKL transcription. Insertion mutants in cbtJKL failed to grow in LB medium, and growth was restored through the addition of cobalt but not other metals. This growth phenotype appeared to be due to the chelation of cobalt present in LB, and cbtJKL mutants also failed to grow in minimal medium containing the chelating agent EDTA unless the medium was supplemented with additional or excess cobalt. In uptake experiments, (57)Co(2+)accumulation was high in wild-type cells expressing the cbtJKL genes, whereas wild-type cells in which cbtJKL expression was repressed showed reduced accumulation. In cbtJKL mutant cells, (57)Co(2+)accumulation was reduced relative to that of the wild type, and presumably, this residual cobalt transport occurred via an alternate ion uptake system(s) that is not specific to cobalt. In symbiosis, the alternate system(s) appeared to mediate cobalt transport into bacteroid cells, as low cbtJKL expression was detected in bacteroids and cbtJKL mutants formed N(2)-fixing nodules on alfalfa.
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