ArticleJournal of bacteriology2011
CT406 encodes a chlamydial ortholog of NrdR, a repressor of ribonucleotide reductase.
Article in Journal of bacteriology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 23 citations in OpenAlex.
- NrdR in Streptococcus and Listeria spp.: DNA Helix Phase Dependence of the Bacterial Ribonucleotide Reductase Repressor.Molecular microbiology · 2025Article
- Molecular pathogenesis ofFrontiers in cellular and infection microbiology · 2023Review
- Article
- GrgA overexpression inhibits Chlamydia trachomatis growth through sigmaMicrobial pathogenesis · 2021Article
- The Repressor Function of theJournal of bacteriology · 2020Article
- A bipartite iron-dependent transcriptional regulation of the tryptophan salvage pathway ineLife · 2019Article
- LC-MS/MS proteomic analysis of starved Bacillus subtilis cells overexpressing ribonucleotide reductase (nrdEF): implications in stress-associated mutagenesis.Current genetics · 2018Article
- Ironing Out the Unconventional Mechanisms of Iron Acquisition and Gene Regulation inFrontiers in cellular and infection microbiology · 2017Review
- NrdR Transcription Regulation: Global Proteome Analysis and Its Role in Escherichia coli Viability and Virulence.PloS one · 2016Article
- Article
- Relevance of the two-component sensor protein CiaH to acid and oxidative stress responses in Streptococcus pyogenes.BMC research notes · 2014Article
- Ribonucleotide reductases: essential enzymes for bacterial life.Frontiers in cellular and infection microbiology · 2014Review
- The early gene product EUO is a transcriptional repressor that selectively regulates promoters of Chlamydia late genes.Molecular microbiology · 2012Article
- Article
- Identification and functional analysis of CT069 as a novel transcriptional regulator in Chlamydia.Journal of bacteriology · 2011Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Chlamydia trachomatis is an obligate intracellular bacterium that is dependent on its host cell for nucleotides. Chlamydia imports ribonucleotide triphosphates (NTPs) but not deoxyribonucleotide triphosphates (dNTPs) and instead uses ribonucleotide reductase to convert imported ribonucleotides into deoxyribonucleotides for DNA synthesis. The genes encoding ribonucleotide reductase have been recently shown to be negatively controlled by a conserved regulator called NrdR. In this study, we provide direct evidence that Escherichia coli NrdR is a transcriptional repressor and that C. trachomatis CT406 encodes its chlamydial ortholog. We showed that CT406 binds specifically to two NrdR boxes upstream of the nrdAB operon in C. trachomatis. Using an in vitro transcription assay, we confirmed that these NrdR boxes function as an operator since they were necessary and sufficient for CT406-mediated repression. We validated our in vitro findings with reporter studies in E. coli showing that both E. coli NrdR and CT406 repressed transcription from the E. coli nrdH and C. trachomatis nrdAB promoters in vivo. This in vivo repression was reversed by hydroxyurea treatment. Since hydroxyurea inhibits ribonucleotide reductase and reduces intracellular deoxyribonucleotide levels, these results suggest that NrdR activity is modulated by a deoxyribonucleotide corepressor.
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