ArticleNucleic acids research2011
Molecular design of a splicing switch responsive to the RNA binding protein Tra2β.
Article in Nucleic acids research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 23 citations in OpenAlex.
- Splicing factor TRA2B enhances synthesis of androgen receptor variant AR-V7 in prostate cancer cells.The Journal of clinical investigation · 2026Article
- Bulk and single-cell alternative splicing analyses reveal roles of TRA2B in myogenic differentiation.Cell proliferation · 2024Article
- Review
- IFI16 directly senses viral RNA and enhances RIG-I transcription and activation to restrict influenza virus infection.Nature microbiology · 2021Article
- 3'untranslated regions (3'UTR) ofAmerican journal of cancer research · 2021Article
- Article
- Expression of TRA2B in endometrial carcinoma and its regulatory roles in endometrial carcinoma cells.Oncology letters · 2019Article
- Human PRPF40B regulates hundreds of alternative splicing targets and represses a hypoxia expression signature.RNA (New York, N.Y.) · 2019Article
- Interplay between coding and exonic splicing regulatory sequences.Genome research · 2019Article
- Roles of silent information regulator 1-serine/arginine-rich splicing factor 10-lipin 1 axis in the pathogenesis of alcohol fatty liver disease.Experimental biology and medicine (Maywood, N.J.) · 2017Review
- Prognostic value of transformer 2β expression in prostate cancer.International journal of clinical and experimental pathology · 2015Article
- Human Tra2 proteins jointly control a CHEK1 splicing switch among alternative and constitutive target exons.Nature communications · 2014Article
- Characterization of the RNA recognition mode of hnRNP G extends its role in SMN2 splicing regulation.Nucleic acids research · 2014Article
- BRCA1 exon 11 a model of long exon splicing regulation.RNA biology · 2014Article
- Neuronal-specific deficiency of the splicing factor Tra2b causes apoptosis in neurogenic areas of the developing mouse brain.PloS one · 2014Article
- The tissue-specific RNA binding protein T-STAR controls regional splicing patterns of neurexin pre-mRNAs in the brain.PLoS genetics · 2013Article
- How does Tra2β protein regulate tissue-specific RNA splicing?Biochemical Society transactions · 2012Review
- Tra2β protein is required for tissue-specific splicing of a smooth muscle myosin phosphatase targeting subunit alternative exon.The Journal of biological chemistry · 2012Article
- Identification of evolutionarily conserved exons as regulated targets for the splicing activator tra2β in development.PLoS genetics · 2011Article
Corrections and comments
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Authors and funding
7 authors at 5 institutions in 2 countries.
Funding
Abstract
Tra2β regulates a number of splicing switches including activation of the human testis-specific exon HIPK3-T in the Homeodomain Interacting Protein Kinase 3 gene. By testing HIPK3-T exons of different intrinsic strengths, we found Tra2β most efficiently activated splicing inclusion of intrinsically weak exons, although these were spliced at a lower overall level. Both the RRM and N-terminal RS-rich region of Tra2β were required for splicing activation. Bioinformatic searches for splicing enhancers and repressors mapped four physically distinct exonic splicing enhancers (ESEs) within HIPK3-T, each containing the known Tra2β AGAA-rich binding site. Surprisingly disruption of each single ESE prevented Tra2β-mediated activation, although single mutated exons could still bind Tra2β protein by gel shifts and functional splicing analyses. Titration experiments indicate an additive model of HIPK3-T splicing activation, requiring availability of an array of four distinct ESEs to enable splicing activation. To enable this efficient Tra2β-mediated splicing switch to operate, a closely adjacent downstream and potentially competitive stronger 5'-splice site is actively repressed. Our data indicate that a novel arrangement of multiple mono-specific AGAA-rich ESEs coupled to a weak 5'-splice site functions as a responsive gauge. This gauge monitors changes in the specific nuclear concentration of the RNA binding protein Tra2β, and co-ordinately regulates HIPK3-T exon splicing inclusion.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.