ArticleMicrobes and infection2011
The effects of macrophage source on the mechanism of phagocytosis and intracellular survival of Leishmania.
Article in Microbes and infection, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 44 citations in OpenAlex.
- Adenosine/AmBio · 2026Article
- Rapid phagosome formation drives parasite control in subclinicalFrontiers in immunology · 2026Article
- Leishmania amazonensis impairs phagosome acidification in B-1 phagocytes as an unrecognized parasite infection and proliferation mechanism.Scientific reports · 2025Article
- Leishmania protein KMP-11 modulates cholesterol transport and membrane fluidity to facilitate host cell invasion.EMBO reports · 2024Article
- Effect of dimethyl fumarate on mitochondrial metabolism in a pediatric porcine model of asphyxia-induced in-hospital cardiac arrest.Scientific reports · 2024Article
- Corrected Super-Resolution Microscopy Enables Nanoscale Imaging of Autofluorescent Lung Macrophages.Biophysical journal · 2020Article
- The macrophage microtubule network acts as a key cellular controller of the intracellular fate of Leishmania infantum.PLoS neglected tropical diseases · 2020Article
- Reactive Oxygen Species Imaging in U937 Cells.Frontiers in physiology · 2020Article
- Techniques to study phagocytosis and uptake of Leishmania tarentolae by J774 macrophages.Experimental parasitology · 2019Article
- Infection of Human Neutrophils WithFrontiers in cellular and infection microbiology · 2019Article
- Quantification of Intracellular Growth Inside Macrophages is a Fast and Reliable Method for Assessing the Virulence of Leishmania Parasites.Journal of visualized experiments : JoVE · 2018Article
- Article
- Cutaneous Manifestations of Human and Murine Leishmaniasis.International journal of molecular sciences · 2017Review
- Lessons from other diseases: granulomatous inflammation in leishmaniasis.Seminars in immunopathology · 2016Review
- Interactions of Francisella tularensis with Alveolar Type II Epithelial Cells and the Murine Respiratory Epithelium.PloS one · 2015Article
- In vitro metacyclogenesis of Leishmania (Viannia) braziliensis and Leishmania (Leishmania) amazonensis clinical field isolates, as evaluated by morphology, complement resistance, and infectivity to human macrophages.BioMed research international · 2015Article
- Cell-to-cell transfer of Leishmania amazonensis amastigotes is mediated by immunomodulatory LAMP-rich parasitophorous extrusions.Cellular microbiology · 2014Article
- Immunoregulation in human American leishmaniasis: balancing pathology and protection.Parasite immunology · 2014Review
- The bacterial septal ring protein RlpA is a lytic transglycosylase that contributes to rod shape and daughter cell separation in Pseudomonas aeruginosa.Molecular microbiology · 2014Article
- Identification and characterization of a gene cluster required for proper rod shape, cell division, and pathogenesis in Clostridium difficile.Journal of bacteriology · 2014Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Leishmania spp. protozoa are obligate intracellular parasites that replicate in macrophages during mammalian infection. Efficient phagocytosis and survival in macrophages are important determinants of parasite virulence. Macrophage lines differ dramatically in their ability to sustain intracellular Leishmania infantum chagasi (Lic). We report that the U937 monocytic cell line supported the intracellular replication and cell-to-cell spread of Lic during 72 h after parasite addition, whereas primary human monocyte-derived macrophages (MDMs) did not. Electron microscopy and live cell imaging illustrated that Lic promastigotes anchored to MDMs via their anterior ends and were engulfed through symmetrical pseudopods. In contrast, U937 cells bound Lic in diverse orientations, and extended membrane lamellae to reorient and internalize parasites through coiling phagocytosis. Lic associated tightly with the parasitophorous vacuole (PV) membrane in both cell types. PVs fused with LAMP-1-expressing compartments 24 h after phagocytosis by MDMs, whereas U937 cell PVs remained LAMP-1 negative. The expression of one phagocytic receptor (CR3) was higher in MDMs than U937 cells, leading us to speculate that parasite uptake proceeds through dissimilar pathways between these cells. We hypothesize that the mechanism of phagocytosis differs between primary versus immortalized human macrophage cells, with corresponding differences in the subsequent intracellular fate of the parasite.
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