ArticleDevelopmental biology2011
Precise cis-regulatory control of spatial and temporal expression of the alx-1 gene in the skeletogenic lineage of s. purpuratus.
Article in Developmental biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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21 citing papers in PubMed, 43 citations in OpenAlex.
- Deep conservation of cis-regulatory elements and chromatin organization in echinoderms uncover ancestral regulatory features of animal genomes.Nature ecology & evolution · 2026Article
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- Transcription of microRNAs is regulated by developmental signaling pathways and transcription factors.Frontiers in cell and developmental biology · 2024Article
- Early expression onset of tissue-specific effector genes during the specification process in sea urchin embryos.EvoDevo · 2023Article
- CRISPR-Cas9 editing of non-coding genomic loci as a means of controlling gene expression in the sea urchin.Developmental biology · 2021Article
- ALX1-related frontonasal dysplasia results from defective neural crest cell development and migration.EMBO molecular medicine · 2020Article
- Cellular bicarbonate accumulation and vesicular proton transport promote calcification in the sea urchin larva.Proceedings. Biological sciences · 2020Article
- Developmental effector gene regulation: Multiplexed strategies for functional analysis.Developmental biology · 2019Article
- From genome to anatomy: The architecture and evolution of the skeletogenic gene regulatory network of sea urchins and other echinoderms.Genesis (New York, N.Y. : 2000) · 2018Review
- Bacterial artificial chromosomes as recombinant reporter constructs to investigate gene expression and regulation in echinoderms.Briefings in functional genomics · 2018Review
- Global analysis of primary mesenchyme cell cis-regulatory modules by chromatin accessibility profiling.BMC genomics · 2018Article
- Article
- Article
- microRNA-31 modulates skeletal patterning in the sea urchin embryo.Development (Cambridge, England) · 2015Article
- Evolutionary rewiring of gene regulatory network linkages at divergence of the echinoid subclasses.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Comparative Study of Regulatory Circuits in Two Sea Urchin Species Reveals Tight Control of Timing and High Conservation of Expression Dynamics.PLoS genetics · 2015Article
- microRNAs regulate β-catenin of the Wnt signaling pathway in early sea urchin development.Developmental biology · 2015Article
- General approach for in vivo recovery of cell type-specific effector gene sets.Genome research · 2014Article
- New regulatory circuit controlling spatial and temporal gene expression in the sea urchin embryo oral ectoderm GRN.Developmental biology · 2013Article
- Spatiotemporal network motif reveals the biological traits of developmental gene regulatory networks in Drosophila melanogaster.BMC systems biology · 2012Article
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2 authors at 1 institution in 1 country.
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Abstract
Deployment of the gene-regulatory network (GRN) responsible for skeletogenesis in the embryo of the sea urchin Strongylocentrotus purpuratus is restricted to the large micromere lineage by a double negative regulatory gate. The gate consists of a GRN subcircuit composed of the pmar1 and hesC genes, which encode repressors and are wired in tandem, plus a set of target regulatory genes under hesC control. The skeletogenic cell state is specified initially by micromere-specific expression of these regulatory genes, viz. alx1, ets1, tbrain and tel, plus the gene encoding the Notch ligand Delta. Here we use a recently developed high throughput methodology for experimental cis-regulatory analysis to elucidate the genomic regulatory system controlling alx1 expression in time and embryonic space. The results entirely confirm the double negative gate control system at the cis-regulatory level, including definition of the functional HesC target sites, and add the crucial new information that the drivers of alx1 expression are initially Ets1, and then Alx1 itself plus Ets1. Cis-regulatory analysis demonstrates that these inputs quantitatively account for the magnitude of alx1 expression. Furthermore, the Alx1 gene product not only performs an auto-regulatory role, promoting a fast rise in alx1 expression, but also, when at high levels, it behaves as an auto-repressor. A synthetic experiment indicates that this behavior is probably due to dimerization. In summary, the results we report provide the sequence level basis for control of alx1 spatial expression by the double negative gate GRN architecture, and explain the rising, then falling temporal expression profile of the alx1 gene in terms of its auto-regulatory genetic wiring.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.