ArticleJournal of clinical pathology1990
In situ hybridisation with digoxigenin-labelled DNA probes for detection of viral genomes.
Article in Journal of clinical pathology, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 60 citations in OpenAlex.
- Localization of annexin I (lipocortin I, p35) mRNA in normal and diseased human skin by in situ hybridization.Archives of dermatological research · 1996Trial
- Distinct biology of Kaposi's sarcoma-associated herpesvirus from primary lesions and body cavity lymphomas.Journal of virology · 1998Article
- Expression of alpha fetoprotein messenger RNA in BEL-7404 human hepatoma cells and effect of L-4 oxalysine on the expression.World journal of gastroenterology · 1998Article
- Detection of parvovirus B19 DNA in bone marrow cells by chemiluminescence in situ hybridization.Journal of clinical microbiology · 1996Article
- A one-day double-labelling technique for tissue specimens: immunogold-silver staining for in situ hybridization combined with alkaline phosphatase-anti-alkaline phosphatase (APAAP) immunohistochemistry for antigens.The Histochemical journal · 1996Article
- The intracellular localization of human cytomegalovirus DNA in peripheral blood leukocytes during active infections by high-resolution fluorescence in situ hybridization.Archives of virology · 1996Article
- The expression of collagen mRNAs in normally developing neonatal rabbit long bones and after treatment of neonatal and adult rabbit tibiae with transforming growth factor-beta 2.The Histochemical journal · 1995Article
- Sensitive detection of human growth hormone mRNA in routinely formalin-fixed, paraffin-embedded transgenic mouse tissues by non-isotopic in situ hybridization.Histochemistry · 1994Article
- The use of 33P-labelled riboprobes for in situ hybridizations: localization of myosin alkali light-chain mRNAs in adult human skeletal muscle.The Histochemical journal · 1994Article
- Association of Epstein-Barr virus with gastric carcinoma with lymphoid stroma.The American journal of pathology · 1993Article
- Comparison of indirect and direct in-situ polymerase chain reaction in cell preparations and tissue sections. Detection of viral DNA, gene rearrangements and chromosomal translocations.Histochemistry · 1993Article
- Detection of Epstein-Barr virus in a case of undifferentiated nasopharyngeal carcinoma by in situ hybridization with digoxigenin-labelled PCR-generated probes.Virchows Archiv. A, Pathological anatomy and histopathology · 1993Article
- Article
- Latent herpes simplex virus type 1 in human geniculate ganglia.Acta neuropathologica · 1992Article
- Molecular characterization of a JC virus (Sap-1) clone derived from a cerebellar form of progressive multifocal leukoencephalopathy.Acta neuropathologica · 1992Article
- Rapid detection of hepatitis B virus DNA in liver tissue by in situ hybridisation and its combination with immunohistochemistry for simultaneous detection of HBV antigens.Journal of clinical pathology · 1991Article
- Detection of single copies of Epstein-Barr virus in paraffin wax sections by non-radioactive in situ hybridisation.Journal of clinical pathology · 1991Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The applicability of a recently developed non-radioactive DNA labelling and detection method, which uses the digoxigenin (DIG) enzyme linked immunosorbent assay (ELISA) system, for the detection of viral infections in pathology specimens by in situ hybridisation, was examined. Its efficacy was compared with that of biotin and radioisotope labelling methods. Three cases of progressive multifocal leucoencephalopathy, two of verruca vulgaris, and seven cases of laryngeal papilloma were studied. The sensitivity of the DIG labelled probe was almost the same as that of a 35S-labelled probe in the dot-blot hybridisation test. Using in situ hybridisation with 35S-labelled and DIG labelled probes, the levels of the hybridised signals detected were similar. The biotin labelled probe was less sensitive, particularly in the cases of laryngeal papilloma. The DIG labelling and detection method was highly sensitive and applicable to the detection of viral infection by ISH, and is preferable to a radiolabelled probe, especially when in situ hybridisation is done in the pathology laboratory.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.