ArticleRadiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology2011
Assessing 'radiosensitivity' with kinetic profiles of γ-H2AX, 53BP1 and BRCA1 foci.
Article in Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Assessment of Individual Radiosensitivity in Breast Cancer Patients Using a Combination of Biomolecular Markers.Biomedicines · 2023Article
- Flow cytometric assessment of DNA double-strand break and repair kinetics in prediction of intrinsic radiosensitivity.Iranian journal of basic medical sciences · 2022Article
- Establishment ofFrontiers in public health · 2022Article
- Ionizing Radiation Protein Biomarkers in Normal Tissue and Their Correlation to Radiosensitivity: A Systematic Review.Journal of personalized medicine · 2021Review
- High-LET Carbon and Iron Ions Elicit a Prolonged and Amplified p53 Signaling and Inflammatory Response Compared to low-LET X-Rays in Human Peripheral Blood Mononuclear Cells.Frontiers in oncology · 2021Article
- Reconstitution of the Ataxia-Telangiectasia Cellular Phenotype With Lentiviral Vectors.Frontiers in immunology · 2018Article
- Clinical and Functional Assays of Radiosensitivity and Radiation-Induced Second Cancer.Cancers · 2017Review
- DNA damage response and hematological malignancy.International journal of hematology · 2017Review
- Compromized DNA repair as a basis for identification of cancer radiotherapy patients with extreme radiosensitivity.Cancer letters · 2016Article
- Identification of novel radiosensitizers in a high-throughput, cell-based screen for DSB repair inhibitors.Molecular cancer therapeutics · 2015Article
- High throughput measurement of γH2AX DSB repair kinetics in a healthy human population.PloS one · 2015Article
- The RABiT: high-throughput technology for assessing global DSB repair.Radiation and environmental biophysics · 2014Article
- Homozygous mutation of MTPAP causes cellular radiosensitivity and persistent DNA double-strand breaks.Cell death & disease · 2014Article
- ATM-dependent MiR-335 targets CtIP and modulates the DNA damage response.PLoS genetics · 2013Article
- XCIND as a genetic disease of X-irradiation hypersensitivity and cancer susceptibility.International journal of hematology · 2013Review
- SMRT compounds abrogate cellular phenotypes of ataxia telangiectasia in neural derivatives of patient-specific hiPSCs.Nature communications · 2013Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
background and purposeDNA repair assays to identify radiosensitive patients have had limited clinical implementation due to long turn-around times or limited specificity. This study evaluates γ-H2AX-Irradiation Induced Foci (IRIF) kinetics as a more rapid surrogate for the 'gold standard' colony survival assay (CSA) using several known DNA repair disorders as reference models. MATERIALS AND
methodsRadiosensitive cells of known and unknown etiology were studied. γ-H2AX-IRIFs were quantified over 24 h, and the curves were fitted by combining logarithmic growth and exponential decay functions. Fitted values that differed from radionormal controls were considered aberrant and compared to CSA results.
resultsWe observed 87% agreement of IRIF data with the CSA for the 14 samples tested. Analysis of γ-H2AX-IRIF kinetics for known repair disorders indicated similarities between an RNF168(-/-) cell line and an RS cell of unknown etiology. These cell lines were further characterized by a reduction in BRCA1-IRIF formation and G2/M checkpoint activation.
conclusionsγ-H2AX-IRIF kinetics showed high concordance with the CSA in RS populations demonstrating its potential as a more rapid surrogate assay. This method provides a means to globally identify defective DNA repair pathways in RS cells of unknown etiology through comparison with known DNA repair defects.
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