ArticleJournal of cellular and molecular medicine2012
Type 2 diabetic obese db/db mice are refractory to myocardial ischaemic post-conditioning in vivo: potential role for Hsp20, F1-ATPase δ and Echs1.
Article in Journal of cellular and molecular medicine, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it, 47 citations in OpenAlex.
- New genetic variants associated with major adverse cardiovascular events in patients with acute coronary syndromes and treated with clopidogrel and aspirin.The pharmacogenomics journal · 2021Pooled it
- Metabolic Syndrome and Efficacy of Remote Ischemic Postconditioning in Acute Moderate Ischemic Stroke: A Post Hoc Analysis of the RICAMIS Trial.Journal of the American Heart Association · 2025Trial
- Effect of hyperglycaemia and diabetes on acute myocardial ischaemia-reperfusion injury and cardioprotection by ischaemic conditioning protocols.British journal of pharmacology · 2020Review
- Long-chain free fatty acids inhibit ischaemic preconditioning of the isolated rat heart.Molecular and cellular biochemistry · 2020Article
- Landscape of heart proteome changes in a diet-induced obesity model.Scientific reports · 2019Article
- Remote ischemic preconditioning fails to reduce infarct size in the Zucker fatty rat model of type-2 diabetes: role of defective humoral communication.Basic research in cardiology · 2018Article
- Adipose tissues of MPC1PeerJ · 2018Article
- Ameliorative potential of conditioning on ischemia-reperfusion injury in diabetes.Conditioning medicine · 2018Article
- Myocyte membrane and microdomain modifications in diabetes: determinants of ischemic tolerance and cardioprotection.Cardiovascular diabetology · 2017Review
- Enoyl-CoA hydratase-1 regulates mTOR signaling and apoptosis by sensing nutrients.Nature communications · 2017Article
- Role of Tissue and Systemic Hypoxia in Obesity and Type 2 Diabetes.Journal of diabetes research · 2016Article
- Article
- Ischaemic conditioning: pitfalls on the path to clinical translation.British journal of pharmacology · 2015Review
- PDE5 inhibitors as therapeutics for heart disease, diabetes and cancer.Pharmacology & therapeutics · 2015Review
- Ischemic conditioning: the challenge of protecting the diabetic heart.Cardiovascular diagnosis and therapy · 2014Review
- Sirtuin 1 (SIRT1) activation mediates sildenafil induced delayed cardioprotection against ischemia-reperfusion injury in mice.PloS one · 2014Article
- Pacing postconditioning: impact of pacing algorithm, gender, and diabetes on its myocardial protective effects.Journal of cardiovascular translational research · 2012Article
- Effects of diabetes on myocardial infarct size and cardioprotection by preconditioning and postconditioning.Cardiovascular diabetology · 2012Review
- Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Ischaemic post-conditioning (PostC) is a clinically relevant cardioprotective modality that has been confirmed in many species including human. It remains unknown if PostC can still protect heart in Type 2 diabetes, a rapidly growing disease in the world. This study investigated the efficacy of PostC in the leptin receptor-deficient db/db mice, which possess Type 2 diabetic characteristics including obesity, hyperglycaemia and hyperleptinaemia. Adult male C57BL/6J wild-type (WT) and db/db mice were anaesthetized, mechanically ventilated and subjected to left coronary artery occlusion for 30 min. followed by 24 hrs of reperfusion. For the PostC groups, the hearts underwent six cycles of 10 sec. of reperfusion and 10 sec. of re-occlusion at the onset of reperfusion. The mice were sacrificed at the end of 24 hrs reperfusion for infarct size measurement. PostC significantly reduced infarct size in WT mice (n = 6/group; P < 0.05), but not in the db/db mice. To identify alterations in protein expression by PostC, proteomic analyses were performed in the heart samples using two-dimensional differential in-gel electrophoresis with three CyDye labelling, followed by mass spectrometry. The results show that mitochondrial proteins (F(1)-ATPase γ and Echs1) were down-regulated by PostC in WT heart. Such change was absent in the db/db heart. On the other hand, PostC reduced Hsp20 in the diabetic heart. In summary, PostC fails to protect Type 2 diabetic mice against ischaemia-reperfusion injury. The potential protein targets for the loss of PostC may include F(1)-ATPase γ, Echs1 and Hsp20 that could regulate cellular ATP consumption/production and defense response to ischaemic stress.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.