ArticleAnalytical chemistry2011
Use of real-time, label-free analysis in revealing low-affinity binding to blood group antigens by Helicobacter pylori.
Article in Analytical chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 47 citations in OpenAlex.
- High sensitive detection of carbohydrate binding proteins in an ELISA-solid phase assay based on multivalent glyconanoparticles.PloS one · 2013Trial
- Fluorophore-conjugated Helicobacter pylori recombinant membrane protein (HopQ) labels primary colon cancer and metastases in orthotopic mouse models by binding CEA-related cell adhesion molecules.Translational oncology · 2020Article
- Promising Recent Strategies with Potential Clinical Translational Value to Combat Antibacterial Resistant Surge.Medicines (Basel, Switzerland) · 2019Review
- Article
- Coating nanoparticles with gastric epithelial cell membrane for targeted antibiotic delivery againstAdvanced therapeutics · 2018Article
- Analysis of binding properties of pathogens and toxins using multivalent glycan microarrays.RSC advances · 2018Article
- Evaluation of Kinetics Using Label-Free Optical Biosensors.Instrumentation science & technology · 2017Article
- Epigallocatechin-3-gallate enhances ER stress-induced cancer cell apoptosis by directly targeting PARP16 activity.Cell death discovery · 2017Article
- Developing an Efficient and General Strategy for Immobilization of Small Molecules onto Microarrays Using Isocyanate Chemistry.Sensors (Basel, Switzerland) · 2016Article
- Structural basis of Lewis(b) antigen binding by the Helicobacter pylori adhesin BabA.Science advances · 2015Article
- Characterization of Receptor Binding Profiles of Influenza A Viruses Using An Ellipsometry-Based Label-Free Glycan Microarray Assay Platform.Biomolecules · 2015Article
- Atomic force microscopy measurements reveal multiple bonds between Helicobacter pylori blood group antigen binding adhesin and Lewis b ligand.Journal of the Royal Society, Interface · 2014Article
- An optics-based variable-temperature assay system for characterizing thermodynamics of biomolecular reactions on solid support.The Review of scientific instruments · 2013Article
- Article
- Carbohydrate microarrays.Chemical Society reviews · 2013Review
- High Throughput, Label-free Screening Small Molecule Compound Libraries for Protein-Ligands using Combination of Small Molecule Microarrays and a Special Ellipsometry-based Optical Scanner.International drug discovery · 2011Article
- Article
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Authors and funding
9 authors at 5 institutions in 4 countries.
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Abstract
Infectious diseases are often initiated by microbial adherence that is mediated by the binding of attachment molecules, termed adhesins, to cell surface receptors on host cells. We present an experimental system, oblique-incidence reflectivity difference (OI-RD) microscopy, which allows the detection of novel, low-affinity microbial attachment mechanisms that may be essential for infectious processes. OI-RD microscopy was used to analyze direct binding of the oncopathogen, Helicobacter pylori ( H. pylori ) to immobilized glycoconjugates in real time with no need for labeling tags. The results suggest the presence of additional Lewis b blood group antigen (Le(b)) binding adhesins that have not been detected previously. OI-RD microscopy also confirmed the high-affinity binding of H. pylori outer-membrane protein BabA to Le(b). The OI-RD microscopy method is broadly applicable to real-time characterization of intact microbial binding to host receptors and offers new strategies to elucidate the molecular interactions of infectious agents with human host cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.