ArticleMolecular imaging and biology2012
Multispectral optoacoustic tomography of matrix metalloproteinase activity in vulnerable human carotid plaques.
Article in Molecular imaging and biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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Who cites it
54 citing papers in PubMed, 108 citations in OpenAlex.
- Optoacoustic muscle imaging.Journal of neuromuscular diseases · 2026Review
- Clinical translation of photoacoustic imaging using exogenous molecular contrast agents [Invited].Biomedical optics express · 2025Review
- Recent Advances in Photoacoustic Imaging: Current Status and Future Perspectives.Micromachines · 2024Review
- Non-Invasive Compression-Induced Anterior Cruciate Ligament (ACL) Injury and In Vivo Imaging of Protease Activity in Mice.Journal of visualized experiments : JoVE · 2023Article
- Cathepsin K-Activated Probe for Fluoro-Photoacoustic Imaging of Early Osteolytic Metastasis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Longitudinal In Vivo Monitoring of Atheroprogression in Hypercholesterolemic Mice Using Photoacoustic Imaging.Thrombosis and haemostasis · 2023Article
- Optoacoustic biomarkers of lipids, hemorrhage and inflammation in carotid atherosclerosis.Frontiers in cardiovascular medicine · 2023Review
- Application of scanning acoustic microscopy for evaluation of MMP activation in multiple cancer cell lines with a smart probe.Turkish journal of biology = Turk biyoloji dergisi · 2023Article
- Noninvasive photoacoustic computed tomography/ultrasound imaging to identify high-risk atherosclerotic plaques.European journal of nuclear medicine and molecular imaging · 2022Article
- Looking deep inside tissue with photoacoustic molecular probes: a review.Journal of biomedical optics · 2022Review
- How molecular imaging will enable robotic precision surgery : The role of artificial intelligence, augmented reality, and navigation.European journal of nuclear medicine and molecular imaging · 2021Review
- Targeted optical fluorescence imaging: a meta-narrative review and future perspectives.European journal of nuclear medicine and molecular imaging · 2021Review
- Multispectral optoacoustic tomography of lipid and hemoglobin contrast in human carotid atherosclerosis.Photoacoustics · 2021Article
- Multi-Scale Imaging of Vascular Pathologies in Cardiovascular Disease.Frontiers in medicine · 2021Review
- New directions of activity-based sensing forChemical science · 2020Review
- Article
- Cardiovascular optoacoustics: From mice to men - A review.Photoacoustics · 2019Review
- Cylindrical illumination with angular coupling for whole-prostate photoacoustic tomography.Biomedical optics express · 2019Article
- Duration of postoperative immobilization affects MMP activity at the healing graft-bone interface: Evaluation in a mouse ACL reconstruction model.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2019Article
- Biodistribution Survey of Oxidized Single-Wall Carbon Nanohorns Following Different Administration Routes by Using Label-Free Multispectral Optoacoustic Tomography.International journal of nanomedicine · 2019Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimsElevated expression of cathepsins, integrins and matrix metalloproteinases (MMPs) is typically associated with atherosclerotic plaque instability. While fluorescent tagging of such molecules has been amply demonstrated, no imaging method was so far shown capable of resolving these inflammation-associated tags with high fidelity and resolution beyond microscopic depths. This study is aimed at demonstrating a new method with high potential for noninvasive clinical cardiovascular diagnostics of vulnerable plaques using high-resolution deep-tissue multispectral optoacoustic tomography (MSOT) technology. METHODS AND
resultsMMP-sensitive activatable fluorescent probe (MMPSense™ 680) was applied to human carotid plaques from symptomatic patients. Atherosclerotic activity was detected by tuning MSOT wavelengths to activation-dependent absorption changes of the molecules, structurally modified in the presence of enzymes. MSOT analysis simultaneously provided morphology along with heterogeneous MMP activity with better than 200 micron resolution throughout the intact plaque tissue. The results corresponded well with epi-fluorescence images made from thin cryosections. Elevated MMP activity was further confirmed by in situ zymography, accompanied by increased macrophage influx.
conclusionsWe demonstrated, for the first time to our knowledge, the ability of MSOT to provide volumetric images of activatable molecular probe distribution deep within optically diffuse tissues. High-resolution mapping of MMP activity was achieved deep in the vulnerable plaque of intact human carotid specimens. This performance directly relates to pre-clinical screening applications in animal models and to clinical decision potential as it might eventually allow for highly specific visualization and staging of plaque vulnerability thus impacting therapeutic clinical decision making.
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