ArticleIntegrative biology : quantitative biosciences from nano to macro2011
Harnessing endogenous growth factor activity modulates stem cell behavior.
Article in Integrative biology : quantitative biosciences from nano to macro, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
35 citing papers in PubMed, 64 citations in OpenAlex.
- Inclusion of Chondroitin Sulfate Into a Gelatin Hydrogel Shifts Local and Global Mechanical Behavior and Fibrochondrogenic Phenotype for Applications in Insertional Tissue Engineering.Journal of biomedical materials research. Part A · 2026Article
- Enhancement of hMSC In Vitro Proliferation by Surface Immobilization of a Heparin-Binding Peptide.Molecules (Basel, Switzerland) · 2023Article
- Material-driven fibronectin and vitronectin assembly enhances BMP-2 presentation and osteogenesis.Materials today. Bio · 2022Article
- Mechanobiological Strategies to Enhance Stem Cell Functionality for Regenerative Medicine and Tissue Engineering.Frontiers in cell and developmental biology · 2021Review
- Heparin-mediated delivery of bone morphogenetic protein-2 improves spatial localization of bone regeneration.Science advances · 2020Article
- Efficient Synthesis of Heparinoid Bioconjugates for Tailoring FGF2 Activity at the Stem Cell-Matrix Interface.Bioconjugate chemistry · 2019Article
- Nanoscale Coatings for Ultralow Dose BMP-2-Driven Regeneration of Critical-Sized Bone Defects.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2019Article
- Incorporating β-cyclodextrin into collagen scaffolds to sequester growth factors and modulate mesenchymal stem cell activity.Acta biomaterialia · 2018Article
- Engineered Coatings for Titanium Implants To Present Ultralow Doses of BMP-7.ACS biomaterials science & engineering · 2018Article
- Competitive Protein Binding Influences Heparin-Based Modulation of Spatial Growth Factor Delivery for Bone Regeneration.Tissue engineering. Part A · 2017Article
- Xeno-Free Strategies for Safe Human Mesenchymal Stem/Stromal Cell Expansion: Supplements and Coatings.Stem cells international · 2017Review
- Material-driven fibronectin assembly for high-efficiency presentation of growth factors.Science advances · 2016Article
- Challenges and Opportunities to Harnessing the (Hematopoietic) Stem Cell Niche.Current stem cell reports · 2016Article
- Tuning cellular responses to BMP-2 with material surfaces.Cytokine & growth factor reviews · 2016Review
- The effect of glycosaminoglycan content on polyethylenimine-based gene delivery within three-dimensional collagen-GAG scaffolds.Biomaterials science · 2015Article
- Design of growth factor sequestering biomaterials.Chemical communications (Cambridge, England) · 2014Review
- Strategies to balance covalent and non-covalent biomolecule attachment within collagen-GAG biomaterials.Biomaterials science · 2014Article
- Heparin microparticle effects on presentation and bioactivity of bone morphogenetic protein-2.Biomaterials · 2014Article
- Chitosan-based scaffolds for bone tissue engineering.Journal of materials chemistry. B · 2014Article
- Materials as stem cell regulators.Nature materials · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
The influence of specific serum-borne biomolecules (e.g. heparin) on growth factor-dependent cell behavior is often difficult to elucidate in traditional cell culture due to the random, non-specific nature of biomolecule adsorption from serum. We hypothesized that chemically well-defined cell culture substrates could be used to study the influence of sequestered heparin on human mesenchymal stem cell (hMSC) behavior. Specifically, we used bio-inert self-assembled monolayers (SAMs) chemically modified with a bioinspired heparin-binding peptide (termed "HEPpep") and an integrin-binding peptide (RGDSP) as stem cell culture substrates. Our results demonstrate that purified heparin binds to HEPpep SAMs in a dose-dependent manner, and serum-borne heparin binds specifically and in a dose-dependent manner to HEPpep SAMs. These heparin-sequestering SAMs enhance hMSC proliferation by amplifying endogenous fibroblast growth factor (FGF) signaling, and enhance hMSC osteogenic differentiation by amplifying endogenous bone morphogenetic protein (BMP) signaling. The effects of heparin-sequestering are similar to the effects of supraphysiologic concentrations of recombinant FGF-2. hMSC phenotype is maintained over multiple population doublings on heparin-sequestering substrates in growth medium, while hMSC osteogenic differentiation is enhanced in a bone morphogenetic protein-dependent manner on the same substrates during culture in osteogenic induction medium. Together, these observations demonstrate that the influence of the substrate on stem cell phenotype is sensitive to the culture medium formulation. Our results also demonstrate that enhanced hMSC proliferation can be spatially localized by patterning the location of HEPpep on the substrate. Importantly, the use of chemically well-defined SAMs in this study eliminated the confounding factor of random, non-specific biomolecule adsorption, and identified serum-borne heparin as a key mediator of hMSC response to endogenous growth factors.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.