ArticleFrontiers in cellular neuroscience2011
Phosphorylation via PKC Regulates the Function of the Drosophila Odorant Co-Receptor.
Article in Frontiers in cellular neuroscience, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed, 105 citations in OpenAlex.
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- Type-specific molecular signaling architectures and synaptic plasticity ofFrontiers in cellular neuroscience · 2025Article
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- Modulation of the NO-cGMP pathway has no effect on olfactory responses in theFrontiers in cellular neuroscience · 2023Article
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- Homeostasis of Mitochondrial CaInsects · 2022Article
- Understanding responses to chemical mixtures: looking forward from the past.Chemical senses · 2022Article
- A Comparative Perspective on Functionally-Related, Intracellular Calcium Channels: The Insect Ryanodine and Inositol 1,4,5-Trisphosphate Receptors.Biomolecules · 2021Review
- PKC98E Regulates Odorant Responses inThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- Impacts of OrX and cAMP-insensitive Orco to the insect olfactory heteromer activity.Molecular biology reports · 2021Article
- Altered functional properties of the codling moth Orco mutagenized in the intracellular loop-3.Scientific reports · 2021Article
- Calmodulin regulates the olfactory performance in Drosophila melanogaster.Scientific reports · 2021Article
- Functional properties of insect olfactory receptors: ionotropic receptors and odorant receptors.Cell and tissue research · 2021Review
- Transduction and Adaptation Mechanisms in the Cilium or Microvilli of Photoreceptors and Olfactory Receptors From Insects to Humans.Frontiers in cellular neuroscience · 2021Review
- A First Glimpse of the Mexican Fruit FlyInternational journal of molecular sciences · 2020Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Insect odorant receptors (ORs) have a unique design of heterodimers formed by an olfactory receptor protein and the ion channel Orco. Heterologously expressed insect ORs are activated via an ionotropic and a metabotropic pathway that leads to cAMP production and activates the Orco channel. The contribution of metabotropic signaling to the insect odor response remains to be elucidated. Disruption of the G(q) protein signaling cascade reduces the odor response (Kain et al., 2008). We investigated this phenomenon in HEK293 cells expressing Drosophila Orco and found that phospholipase C (PLC) inhibition reduced the sensitivity of Orco to cAMP. A similar effect was seen upon inhibition of protein kinase C (PKC), whereas PKC stimulation activated Orco even in the absence of cAMP. Mutation of the five PKC phosphorylation sites in Orco almost completely eliminated sensitivity to cAMP. To test the impact of PKC activity in vivo we combined single sensillum electrophysiological recordings with microinjection of agents affecting PLC and PKC function and observed an altered response of olfactory sensory neurons (OSNs) to odorant stimulation. Injection of the PLC inhibitor U73122 or the PKC inhibitor Gö6976 into sensilla reduced the OSN response to odor pulses. Conversely, injection of the PKC activators OAG, a diacylglycerol analog, or phorbol myristate acetate (PMA) enhanced the odor response. We conclude that metabotropic pathways affecting the phosphorylation state of Orco regulate OR function and thereby shape the OSN odor response.
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