ArticleNature protocols2011
Reproducible subcutaneous transplantation of cell sheets into recipient mice.
Article in Nature protocols, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed.
- Chitin nanofibers: recent advances in preparation and applications in biomedical and beyond.RSC advances · 2025Review
- Mouse Models of Renal Allograft Transplant Rejection: Methods to Investigate Chemokine-GAG Interaction and Therapeutic Blockade.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Antibacterial, pro-angiogenic and pro-osteointegrative zein-bioactive glass/copper based coatings for implantable stainless steel aimed at bone healing.Bioactive materials · 2021Article
- Comparison of mesenchymal stem cell sheets and chondrocyte sheets in a rabbit growth plate injury modelTurkish journal of medical sciences · 2020Article
- Stem Cell Pathology.Annual review of pathology · 2018Review
- Targeted Genome Engineering to Control VEGF Expression in Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells: Potential Implications for the Treatment of Myocardial Infarction.Stem cells translational medicine · 2017Article
- Retraction: Reproducible subcutaneous transplantation of cell sheets into recipient mice.Nature protocols · 2016Article
- In Vivo Experiments with Dental Pulp Stem Cells for Pulp-Dentin Complex Regeneration.Mediators of inflammation · 2015Review
- Layer-by-layer assembly of 3D tissue constructs with functionalized graphene.Advanced functional materials · 2014Article
- Endothelial cells enhance the in vivo bone-forming ability of osteogenic cell sheets.Laboratory investigation; a journal of technical methods and pathology · 2014Article
- Preparation of three-dimensional vascularized MSC cell sheet constructs for tissue regeneration.BioMed research international · 2014Article
- Proangiogenic microtemplated fibrin scaffolds containing aprotinin promote improved wound healing responses.Angiogenesis · 2014Article
- Chitin Nanofiber Micropatterned Flexible Substrates for Tissue EngineeringJournal of materials chemistry. B · 2013Article
- Genetically modified adipose tissue-derived stem/stromal cells, using simian immunodeficiency virus-based lentiviral vectors, in the treatment of hemophilia B.Human gene therapy · 2013Article
- Brief report: Mechanism of extravasation of infused stem cells.Stem cells (Dayton, Ohio) · 2012Article
- Article
Corrections and comments
- Retraction · 2016-01-13Concerns/Issues about Data · Concerns/Issues about Image · Concerns/Issues about Results and/or Conclusions ·
- Retracted
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Perfecting tissue engineering and cell sheet transplantation is an important step toward realizing regenerative medicine and is a growing area of research. Before being applied to clinical settings, it is important that these approaches are evaluated in vivo. Here we provide a detailed protocol for handling thin cell sheets, for a simple and highly reproducible subcutaneous transplantation of cell sheets into mice, and for the histological examination of regenerated tissues. Various aspects of transplants can be assessed, such as maintenance, differentiation and proliferation. An emphasis is placed on surgical precision and reproducibility. The resulting consistency between surgeries helps minimize artifacts from surgical variation and therefore enables researchers to not only observe and compare the interactions between host tissues but also to compare transplants among different host animals. A single transplantation can be carried out within ∼10 min.
Indexed as
Identifiers
21720318What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.