ArticleJournal of the American Society of Nephrology : JASN2011
Multiphoton imaging of the functioning kidney.
Article in Journal of the American Society of Nephrology : JASN, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 46 citations in OpenAlex.
- Autofluorescence spectroscopy and multispectral autofluorescence microscopy for characterization of lupus nephritis in renal tissues.Scientific reports · 2026Article
- Innovative Technologies for Kidney Research: Three-Dimensional Imaging and Quantification.Seminars in nephrology · 2026Review
- An autopsy case with tubular obstruction by impacted swollen blebs due to ischemic acute kidney injury.BMC nephrology · 2025Article
- Viscocohesive hyaluronan gel enhances stability of intravital multiphoton imaging with subcellular resolution.Neurophotonics · 2025Article
- Mapping single-nephron filtration in the isolated, perfused rat kidney using magnetic resonance imaging.American journal of physiology. Renal physiology · 2022Article
- Advances in fluorescence microscopy techniques to study kidney function.Nature reviews. Nephrology · 2021Review
- Monitoring kidney optical properties during cold storage preservation with spatial frequency domain imaging.Journal of biomedical optics · 2019Article
- Early loss of mitochondrial complex I and rewiring of glutathione metabolism in renal oncocytoma.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Kidney Imaging: Intravital Microscopy.Methods in molecular biology (Clifton, N.J.) · 2018Article
- Intravital imaging of the kidney.Methods (San Diego, Calif.) · 2017Article
- The Mouse Isolated Perfused Kidney Technique.Journal of visualized experiments : JoVE · 2016Article
- Deep insights: intravital imaging with two-photon microscopy.Pflugers Archiv : European journal of physiology · 2016Review
- Cysteine S-Glutathionylation Promotes Stability and Activation of the Hippo Downstream Effector Transcriptional Co-activator with PDZ-binding Motif (TAZ).The Journal of biological chemistry · 2016Article
- Autophagy, Innate Immunity and Tissue Repair in Acute Kidney Injury.International journal of molecular sciences · 2016Review
- Just Look! Intravital Microscopy as the Best Means to Study Kidney Cell Death Dynamics.Seminars in nephrology · 2016Review
- Design and performance of an ultra-flexible two-photon microscope for in vivo research.Biomedical optics express · 2015Article
- Novel in vivo techniques to visualize kidney anatomy and function.Kidney international · 2015Review
- Dynamic multiphoton microscopy: focusing light on acute kidney injury.Physiology (Bethesda, Md.) · 2014Review
- Epac-Rap signaling reduces oxidative stress in the tubular epithelium.Journal of the American Society of Nephrology : JASN · 2014Article
- Mitochondrial reactive oxygen species (ROS) and ROS-induced ROS release.Physiological reviews · 2014Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Translating discoveries made in isolated renal cells and tubules to the in vivo situation requires the assessment of cellular function in intact live organs. Multiphoton imaging is a form of fluorescence microscopy that is ideally suited to working with whole tissues and organs, but adequately loading cells with fluorescence dyes in vivo remains a challenge. We found that recirculation of fluorescence dyes in the rat isolated perfused kidney (IPK) resulted in levels of intracellular loading that would be difficult to achieve in vivo. This technique allowed the imaging of tubular cell structure and function with multiphoton microscopy in an intact, functioning organ. We used this approach to follow processes in real time, including (1) relative rates of reactive oxygen species (ROS) production in different tubule types, (2) filtration and tubular uptake of low-molecular-weight dextrans and proteins, and (3) the effects of ischemia-reperfusion injury on mitochondrial function and cell structure. This study demonstrates that multiphoton microscopy of the isolated perfused kidney is a powerful technique for detailed imaging of cell structure and function in an intact organ.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.