Evidence map›Paper›PMID 21718699›Full record

ArticleJournal of immunological methods2011

Synergetic chemiluminescence and label-free dual detection for developing a hepatitis protein array.

C Pereira, A Yalçın, M Cretich, M Chiari, M S Unlü, D Nunes, D A Bergstein

Abstract readEvaluation Study
In one paragraph

Article in Journal of immunological methods, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

C PereiraZoiray Technologies Inc., Boston, MA, USA.
A Yalçın
M Cretich
M Chiari
M S Unlü
D Nunes
D A Bergstein

Funding

Label-free Multiplexed Immunoassay Platform for Diagnosing Liver DiseaseR43DK083167 · NIDDK · ZOIRAY TECHNOLOGIES, INC. · PI BERGSTEIN, DAVID ALAN · 2009 to 2009
$178k
NIDDK NIH HHS 1R43DK083167-01NIDDK NIH HHS R43 DK083167
6 · The paper itself

Abstract

A dual detection system for protein arrays is presented that combines label-free detection by optical interference with chemiluminescence. A planar protein array that targets hepatitis B surface antigen is developed. Surface densities for individual antibody spots are quantitated using optical interference prior to use. Target binding (10 ng/ml) is detected label-free. Target binding (1 ng/ml) is detected by both optical interference and chemiluminescence with the inclusion of secondary antibodies. Binding results using both methods are found to be directly proportion to the capture probe density measured initially. The dual detection system provides the analytical utility of optical interference detection with the established clinical utility of chemiluminescence detection.

Indexed as

Hepatitis BHepatitis B AntibodiesHepatitis B Surface AntigensHumansImmunoassayInterferometryLuminescent MeasurementsProtein Array AnalysisHepatitis B AntibodiesHepatitis B Surface Antigens

Identifiers

PMID21718699
PMCPMC3152660

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.