ArticlePlant methods2011
sunTILL: a TILLING resource for gene function analysis in sunflower.
Article in Plant methods, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- EMS-induced genomic variation and QTL-seq analysis of safflower spininess through whole genome sequencing (WGS).BMC genomics · 2025Article
- Article
- Chemical-induced variations in callus regeneratedBiotechnology reports (Amsterdam, Netherlands) · 2025Article
- Directed mutagenesis in fruit crops.3 Biotech · 2025Review
- Genetic Screening of Factors in the Plant Protein Secretion.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Is it the end of TILLING era in plant science?Frontiers in plant science · 2023Review
- Current trends and insights on EMS mutagenesis application to studies on plant abiotic stress tolerance and development.Frontiers in plant science · 2022Review
- A TILLING by sequencing approach to identify induced mutations in sunflower genes.Scientific reports · 2021Article
- Sunflower inflorescences absorb maximum light energy if they face east and afternoons are cloudier than mornings.Scientific reports · 2020Article
- Deployment of Genetic and Genomic Tools Toward Gaining a Better Understanding of Rice-Frontiers in plant science · 2020Review
- Identification of induced mutations in hexaploid wheat genome using exome capture assay.PloS one · 2018Article
- Sunflower Hybrid Breeding: From Markers to Genomic Selection.Frontiers in plant science · 2017Review
- COTIP: Cotton TILLING Platform, a Resource for Plant Improvement and Reverse Genetic Studies.Frontiers in plant science · 2016Article
- Sunflower Resistance to Broomrape (Orobanche cumana) Is Controlled by Specific QTLs for Different Parasitism Stages.Frontiers in plant science · 2016Article
- Tomato Fruits Show Wide Phenomic Diversity but Fruit Developmental Genes Show Low Genomic Diversity.PloS one · 2016Article
- PT-Flax (phenotyping and TILLinG of flax): development of a flax (Linum usitatissimum L.) mutant population and TILLinG platform for forward and reverse genetics.BMC plant biology · 2013Article
- SMART--Sunflower Mutant population And Reverse genetic Tool for crop improvement.BMC plant biology · 2013Article
- Development and characterization of a new TILLING population of common bread wheat (Triticum aestivum L.).PloS one · 2012Article
- Mutagenesis as a tool in plant genetics, functional genomics, and breeding.International journal of plant genomics · 2011Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCultivated sunflower (Helianthus annus L.) is a globally important oilseed crop, subjected to intensive genetic and genomic studies. Although classical mutagenesis has successfully been applied to Helianthus genus in the past, we have developed the first sunflower TILLING resource.
resultsTo balance the maximum mutation density with an acceptable plant survival rate, a 'kill curve' analysis was first conducted with different ethylmethanesulfonate (EMS) dosages and different exposure times. According to the germination rate, a treatment with 0.7% EMS for 6 h was chosen. An M2 progeny of 3,651 fertile plants was obtained. Totally, 4.79% of the whole population showed clear aberrant phenotypes. A microsatellite analysis on a representative sample of the original seed stock and mutant lines confirmed the uniformity of the genetic background of plant material. The TILLING procedure was successfully applied to sunflower genome, initially by a CelI-nuclease mismatch cleavage assay coupled with a DNA-pooling level test. To investigate the efficiency of the mutagenic treatment, a pilot screening was carried out on 1,152 M2 lines focusing on four genes, three involved in the fatty acid biosynthetic pathway and one for downy mildew resistance. A total of 9 mutant lines were identified and confirmed by sequencing; thereby, the estimated overall mutation frequency for the pilot assay resulted to be 1/475 kb.
conclusionA first TILLING population for a high throughput identification of EMS-induced point mutations in sunflower genome has been successfully obtained. This represents a powerful tool to a better understanding of gene function in sunflower.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.