ArticlePlant methods2011
A novel procedure for the quantitative analysis of metabolites, storage products and transcripts of laser microdissected seed tissues of Brassica napus.
Article in Plant methods, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Integrating Large-Scale Data and RNA Technology to Protect Crops from Fungal Pathogens.Frontiers in plant science · 2016Review
- Laser Microdissection and Spatiotemporal Pinoresinol-Lariciresinol Reductase Gene Expression Assign the Cell Layer-Specific Accumulation of Secoisolariciresinol Diglucoside in Flaxseed Coats.Frontiers in plant science · 2016Article
- Metabolic Architecture of the Cereal Grain and Its Relevance to Maximize Carbon Use Efficiency.Plant physiology · 2015Article
- Void space inside the developing seed of Brassica napus and the modelling of its function.The New phytologist · 2013Article
- Seed architecture shapes embryo metabolism in oilseed rape.The Plant cell · 2013Article
- A noninvasive platform for imaging and quantifying oil storage in submillimeter tobacco seed.Plant physiology · 2013Article
- Transcriptome-wide mapping of pea seed ageing reveals a pivotal role for genes related to oxidative stress and programmed cell death.PloS one · 2013Article
- Tissue-specific distribution of secondary metabolites in rapeseed (Brassica napus L.).PloS one · 2012Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe biology of the seed is complicated by the extensive non-homogeneity (spatial gradients) in gene expression, metabolic conversions and storage product accumulation. The detailed understanding of the mechanisms underlying seed growth and storage therefore requires the development of means to obtain tissue-specific analyses. This approach also represents an important priority in the context of seed biotechnology.
resultsWe provide a guideline and detailed procedures towards the quantitative analysis of laser micro-dissected (LM) tissues in oilseed rape (Brassica napus). This includes protocols for laser microdissection of the seed, and the subsequent extraction and quantitative analysis of lipids, starch and metabolites (sugars, sugar phosphates, nucleotides, amino acids, intermediates of glycolysis and citric acid cycle). We have also developed a protocol allowing the parallel analysis of the transcriptome using Brassica-specific microarrays. Some data are presented regarding the compartmentation of metabolites within the oilseed rape embryo.
conclusionThe described methodology allows for the rapid, combined analysis of metabolic intermediates, major storage products and transcripts in a tissue-specific manner. The protocols are robust for oilseed rape, and should be readily adjustable for other crop species. The suite of methods applied to LM tissues represents an important step in the context of both the systems biology and the biotechnology of oilseeds.
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Registered trials
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