ArticleBMC research notes2011
Characterisation of five candidate genes within the ETEC F4ab/ac candidate region in pigs.
Article in BMC research notes, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 24 citations in OpenAlex.
- Detection and Analysis of Antidiarrheal Genes and Immune Factors in Various Shanghai Pig Breeds.Biomolecules · 2024Article
- SNP discovery and association study for growth, fatness and meat quality traits in Iberian crossbred pigs.Scientific reports · 2022Article
- Effects of a microencapsulated formula of organic acids and essential oils on nutrient absorption, immunity, gut barrier function, and abundance of enterotoxigenic Escherichia coli F4 in weaned piglets challenged with E. coli F4.Journal of animal science · 2020Article
- Association of3 Biotech · 2019Article
- Resistance to ETEC F4/F18-mediated piglet diarrhoea: opening the gene black box.Tropical animal health and production · 2019Review
- Effect of Puerarin, Baicalin and Berberine Hydrochloride on the Regulation of IPEC-J2 Cells Infected with EnterotoxigenicEvidence-based complementary and alternative medicine : eCAM · 2019Article
- Binding determinants in the interplay between porcine aminopeptidase N and enterotoxigenic Escherichia coli F4 fimbriae.Veterinary research · 2018Article
- Porcine aminopeptidase N binds to F4+ enterotoxigenic Escherichia coli fimbriae.Veterinary research · 2016Article
- Gene silencing of porcine MUC13 and ITGB5: candidate genes towards Escherichia coli F4ac adhesion.PloS one · 2013Article
- Immunogenomics for identification of disease resistance genes in pigs: a review focusing on Gram-negative bacilli.Journal of animal science and biotechnology · 2012Article
- The search for the gene mutations underlying enterotoxigenic Escherichia coli F4ab/ac susceptibility in pigs: a review.Veterinary research · 2012Review
- Differential gene expression profiling of porcine epithelial cells infected with three enterotoxigenic Escherichia coli strains.BMC genomics · 2012Article
- Nucleotide variability and linkage disequilibrium patterns in the porcine MUC4 gene.BMC genetics · 2012Article
- Susceptibility towards enterotoxigenic Escherichia coli F4ac diarrhea is governed by the MUC13 gene in pigs.PloS one · 2012Article
Corrections and comments
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Authors and funding
15 authors at 7 institutions in 5 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundEnterotoxigenic Escherichia coli (ETEC) that express the F4ab and F4ac fimbriae is a major contributor to diarrhoea outbreaks in the pig breeding industry, infecting both newborn and weaned piglets. Some pigs are resistant to this infection, and susceptibility is inherited as a simple dominant Mendelian trait. Indentifying the genetics behind this trait will greatly benefit pig welfare as well as the pig breeding industry by providing an opportunity to select against genetically susceptible animals, thereby reducing the number of diarrhoea outbreaks. The trait has recently been mapped by haplotype sharing to a 2.5 Mb region on pig chromosome 13, a region containing 18 annotated genes.
findingsThe coding regions of five candidate genes for susceptibility to ETEC F4ab/ac infection (TFRC, ACK1, MUC20, MUC4 and KIAA0226), all located in the 2.5 Mb region, were investigated for the presence of possible causative mutations. A total of 34 polymorphisms were identified in either coding regions or their flanking introns. The genotyping data for two of those were found to perfectly match the genotypes at the ETEC F4ab/ac locus, a G to C polymorphism in intron 11 of TFRC and a C to T silent polymorphism in exon 22 of KIAA0226. Transcriptional profiles of the five genes were investigated in a porcine tissue panel including various intestinal tissues. All five genes were expressed in intestinal tissues at different levels but none of the genes were found differentially expressed between ETEC F4ab/ac resistant and ETEC F4ab/ac susceptible animals in any of the tested tissues.
conclusionsNone of the identified polymorphisms are obvious causative mutations for ETEC F4ab/ac susceptibility, as they have no impact on the level of the overall mRNA expression nor predicted to influence the composition of the amino acids composition. However, we cannot exclude that the five tested genes are bona fide candidate genes for susceptibility to ETEC F4ab/ac infection since the identified polymorphism might affect the translational apparatus, alternative splice forms may exist and post translational mechanisms might contribute to disease susceptibility.
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Registered trials
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