ArticleAnalytical chemistry2011
Molecular detection of bacterial pathogens using microparticle enhanced double-stranded DNA probes.
Article in Analytical chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Fluorescent primers amplification refractory mutation system qPCR (FP ARMS-qPCR) for MTHFR C677T SNP genotyping.Molecular biology reports · 2024Article
- Development of Flow Cytometric Assay for Detecting Papillary Thyroid Carcinoma Related hsa-miR-146b-5p through Toehold-Mediated Strand Displacement Reaction on Magnetic Beads.Molecules (Basel, Switzerland) · 2021Article
- Rapid Detection of Hepatitis B Virus in Blood Samples Using a Combination of Polymerase Spiral Reaction With Nanoparticles Lateral-Flow Biosensor.Frontiers in molecular biosciences · 2020Article
- Nanotube assisted microwave electroporation for single cell pathogen identification and antimicrobial susceptibility testing.Nanomedicine : nanotechnology, biology, and medicine · 2019Article
- Simultaneous and Ultrasensitive Detection of Foodborne Bacteria by Gold Nanoparticles-Amplified Microcantilever Array Biosensor.Frontiers in chemistry · 2019Article
- Synthesis of Fluorescence Turn-On DNA Hybridization Probe Using theCurrent protocols in nucleic acid chemistry · 2018Article
- A Rapid and Low-Cost Pathogen Detection Platform by Using a Molecular Agglutination Assay.ACS central science · 2018Article
- A highly efficient bead extraction technique with low bead number for digital microfluidic immunoassay.Biomicrofluidics · 2016Article
- Efficient SNP Discovery by Combining Microarray and Lab-on-a-Chip Data for Animal Breeding and Selection.Microarrays (Basel, Switzerland) · 2015Review
- Lab on a single microbead: an ultrasensitive detection strategy enabling microRNA analysis at the single-molecule level.Chemical science · 2015Article
- Composite generalized Langevin equation for Brownian motion in different hydrodynamic and adhesion regimes.Physical review. E, Statistical, nonlinear, and soft matter physics · 2015Article
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- Rapid antimicrobial susceptibility testing with electrokinetics enhanced biosensors for diagnosis of acute bacterial infections.Annals of biomedical engineering · 2014Article
- Single cell gene expression analysis in injury-induced collective cell migration.Integrative biology : quantitative biosciences from nano to macro · 2014Article
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- An on-chip, multichannel droplet sorter using standing surface acoustic waves.Analytical chemistry · 2013Article
Corrections and comments
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5 authors.
Funding
Abstract
Rapid, specific, and sensitive detection of bacterial pathogens is essential toward clinical management of infectious diseases. Traditional approaches for pathogen detection, however, often require time-intensive bacterial culture and amplification procedures. Herein, a microparticle enhanced double-stranded DNA probe is demonstrated for rapid species-specific detection of bacterial 16S rRNA. In this molecular assay, the binding of the target sequence to the fluorophore conjugated probe thermodynamically displaces the quencher probe and allows the fluorophore to fluoresce. By incorporation of streptavidin-coated microparticles to localize the biotinylated probes, the sensitivity of the assay can be improved by 3 orders of magnitude. The limit of detection of the assay is as few as eight bacteria without target amplification and is highly specific against other common pathogens. Its applicability toward clinical diagnostics is demonstrated by directly identifying bacterial pathogens in urine samples from patients with urinary tract infections.
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Registered trials
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