ArticleIn vitro cellular & developmental biology. Animal2011
Different doses of bone morphogenetic protein 4 promote the expression of early germ cell-specific gene in bone marrow mesenchymal stem cells.
Article in In vitro cellular & developmental biology. Animal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 1 of them a synthesis that pooled it.
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Who cites it
22 citing papers in PubMed, 1 synthesis or guideline pooled it, 43 citations in OpenAlex.
- Mesenchymal stromal/stem cells and their exosomes for restoration of spermatogenesis in non-obstructive azoospermia: a systemic review.Stem cell research & therapy · 2021Pooled it
- Enhancing Spermatogenesis in Non-obstructive Azoospermia Through Mesenchymal Stem Cell Therapy22.Current stem cell research & therapy · 2024Review
- The Effect of miR-106b-5p Expression in The Production of iPS-Like Cells from Mice SSCs during The Formation of Teratoma and The Three Embryonic Layers.Cell journal · 2022Article
- Strategies for Mammalian Mesenchymal Stem Cells Differentiation into Primordial Germ Cell-Like Cells: A Review.Cell journal · 2022Article
- Comparison of the efficacy of bone morphogenetic protein-4 onResearch in pharmaceutical sciences · 2022Article
- Article
- Analysis of MiRNA-17 and MiRNA-146 Expression During Differentiation of Spermatogonial Stem Like Cells Derived from Mouse Bone Marrow Mesenchymal Stem Cells.International journal of molecular and cellular medicine · 2019Article
- Evaluation of specific germ cell genes expression in mouse embryonic stem cell-derived germ cell like cells treated with bone morphogenetic protein 4 in vitro.International journal of reproductive biomedicine · 2018Article
- Pericyte-derived bone morphogenetic protein 4 underlies white matter damage after chronic hypoperfusion.Brain pathology (Zurich, Switzerland) · 2018Article
- Proliferation in culture of primordial germ cells derived from embryonic stem cell: induction by retinoic acid.Bioscience reports · 2016Article
- Amniotic membrane mesenchymal stem cells can differentiate into germ cells in vitro.In vitro cellular & developmental biology. Animal · 2016Article
- Primordial germ cell-like cells derived from canine adipose mesenchymal stem cells.Cell proliferation · 2016Article
- Overexpression of CD61 promotes hUC-MSC differentiation into male germ-like cells.Cell proliferation · 2016Article
- Derivation of male germ cells from ram bone marrow mesenchymal stem cells by three different methods and evaluation of their fate after transplantation into the testis.In vitro cellular & developmental biology. Animal · 2016Article
- Temporal expression of calcium channel subunits in satellite cells and bone marrow mesenchymal cells.Stem cell reviews and reports · 2015Article
- BMP4 promotes SSEA-1(+) hUC-MSC differentiation into male germ-like cells in vitro.Cell proliferation · 2014Article
- Comparison of differentiation potential of male mouse adipose tissue and bone marrow derived-mesenchymal stem cells into germ cells.Iranian journal of reproductive medicine · 2013Article
- Influence of activin A supplementation during human embryonic stem cell derivation on germ cell differentiation potential.Stem cells and development · 2013Article
- Investigation on the effect of static magnetic field up to 15 mT on the viability and proliferation rate of rat bone marrow stem cells.In vitro cellular & developmental biology. Animal · 2013Article
- Overexpression of bone morphogenetic protein 4 in STO fibroblast feeder cells represses the proliferation of mouse embryonic stem cells in vitro.Experimental & molecular medicine · 2012Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bone morphogenetic protein (BMP)-4 has a crucial role on primordial germ cells (PGCs) development in vivo which can promote stem cell differentiation to PG-like cells. In this study, we investigated the expression of Mvh as one of the specific genes in primordial germ cells after treatment with different doses of BMP4 on bone mesenchymal stem cells (BMSCs)-derived PGCs. Following isolation of BMSCs from male mouse femur and tibia, cells were cultured in medium for 72 h. Passage 4 murine BMSCs were characterized by CD90, CD105, CD34, and CD45 markers and osteo-adipogenic differentiation. Different doses of BMP4 (0, 0.01, 0.1, 1, 5, 25, 50, and 100 ng/ml) were added to BMSCs for PGCs differentiation during 4-days culture. Viability percent, proliferation rates, and expression of Mvh gene were analyzed by RT-qPCR. Data analysis was done with ANOVA test. CD90(+), CD105(+), CD34(-), and CD45(-) BMSCs were able to differentiate to osteo-adipogenic lineages. The results revealed that proliferation rate and viability percent were raised significantly (p ≤ 0.05) by adding 1, 5, 25 ng/ml of BMP4 and there were decreased to the lowest rate after adding 100 ng/ml BMP4 (p ≤ 0.05). There were significant up regulation (p ≤ 0.05) in Mvh expression between 25, 50, and 100 ng/ml BMP4 with other doses. So the selective dose of BMP-4 for treatment during 4-day culture was 25 ng/ml. The results suggest that addition of 25 ng/ml BMP4 had the best effects based on gene-specific marker expression.
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