ArticlePlant cell reports2011
An efficient Agrobacterium-mediated transformation and regeneration system for leaf explants of two elite aspen hybrid clones Populus alba × P. berolinensis and Populus davidiana × P. bolleana.
Article in Plant cell reports, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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37 citing papers in PubMed, 103 citations in OpenAlex.
- Establishment of an efficient and reliable Agrobacterium-mediated genetic transformation system for the elite male-sterile poplar 'Siyang-1'.BMC plant biology · 2026Article
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- Overexpression of PtrCWINV2 accelerates shoot growth and wood development in hybrid poplar (Populus davidiana × Populus bolleana).Plant cell reports · 2025Article
- Genome-Wide Identification and Functional Analysis ofInternational journal of molecular sciences · 2025Article
- Poplar transformation with variable explant sources to maximize transformation efficiency.Scientific reports · 2025Article
- A faster killing effect of plastid-mediated RNA interference on a leaf beetle through induced dysbiosis of the gut bacteria.Plant communications · 2024Article
- RtNAC055 promotes drought tolerance via a stomatal closure pathway linked to methyl jasmonate/hydrogen peroxide signaling inHorticulture research · 2024Article
- Optimization of High-Efficiency Tissue Culture Regeneration Systems in Gray Poplar.Life (Basel, Switzerland) · 2023Article
- Uncovering the Role of PdePrx12 Peroxidase in Enhancing Disease Resistance in Poplar Trees.Journal of fungi (Basel, Switzerland) · 2023Article
- Effect of a suitable treatment period on the genetic transformation efficiency of the plant leaf disc method.Plant methods · 2023Article
- Review
- Application of a novel strong promoter from Chinese fir (Frontiers in plant science · 2023Article
- The GlutathioneInternational journal of molecular sciences · 2022Article
- An EfficientInternational journal of molecular sciences · 2022Article
- Article
- Article
- The Receptor-Like KinaseInternational journal of molecular sciences · 2021Article
- An improved and efficient method of Agrobacterium syringe infiltration for transient transformation and its application in the elucidation of gene function in poplar.BMC plant biology · 2021Article
- Generation of a Golden Leaf Triploid Poplar by Repressing the Expression ofForestry research · 2021Article
- Drought Stress-Mediated Transcriptome Profile Reveals NCED as a Key Player Modulating Drought Tolerance inFrontiers in plant science · 2021Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transgenic technology has been successfully used for gene function analyses and trait improvement in cereal plants. However, its usage is limited in woody plants, especially in the difficult-to-transform but commercially viable hybrid poplar. In this work, an efficient regeneration and transformation system was established for the production of two hybrid aspen clones: Populus alba × P. berolinensis and Populus davidiana × P. bolleana. A plant transformation vector designed to express the reporter gene uidA, encoding β-glucuronidase (GUS), driven by the cauliflower mosaic virus 35S promoter, was used to detect transformation event at early stages of plant regeneration, and to optimize the parameters that may affect poplar transformation efficiency. Bacterium strain and age of leaf explant are two major factors that affect transformation efficiency. Addition of thidiazuron (TDZ) improved both regeneration and transformation efficiency. The transformation efficiency is approximately 9.3% for P. alba × P. berolinensis and 16.4% for P. davidiana × P. bolleana. Using this system, transgenic plants were usually produced in less than 1 month after co-cultivation. The growth characteristics and morphology of transgenic plants were identical to the untransformed wild type plants, and the transgenes could be inherited by vegetative propagation, as confirmed by PCR, Southern blotting, RT-PCR and β-glucuronidase staining analyses. The establishment of this system will help to facilitate the studies of gene functions in tree growth and development at a genome level, and as well as the introduction of some valuable traits in aspen breeding.
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