ArticleInternational journal of legal medicine2012
Comparison of three methods of DNA extraction from human bones with different degrees of degradation.
Article in International journal of legal medicine, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Bone Marrow as a Source of DNA in Forensic Genetics: An Optimized Nucleic Acids Extraction Protocol.Genes · 2026Article
- Computed tomography of the occipital bone for sex estimation in Turks.Revista da Associacao Medica Brasileira (1992) · 2026Article
- Influence of the amount of saliva deposition and time elapsed after deposition on bite mark analysis.Forensic science, medicine, and pathology · 2024Article
- Optimization of InnoXtract™ extraction and purification system for DNA extraction from skeletal samples.International journal of legal medicine · 2023Article
- DNA Extraction of Bone Through Demineralization.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Suitability of specific soft tissue swabs for the forensic identification of highly decomposed bodies.International journal of legal medicine · 2021Article
- Improved autosomal STR typing of degraded femur samples extracted using a custom demineralization buffer and DNA IQ™.Forensic science international. Synergy · 2021Article
- Fragment analysis in forensic anthropology.Forensic sciences research · 2020Review
- Article
- DNA typing from skeletal remains: a comparison between capillary electrophoresis and massively parallel sequencing platforms.International journal of legal medicine · 2020Article
- A novel, 4-h DNA extraction method for STR typing of casework bone samples.International journal of legal medicine · 2020Article
- DNA Purification Cell Lysis and Wash Step Modifications for Low-Template DNA Sample ProcessingJournal of forensic sciences · 2020Article
- Article
- Optimization of DNA extraction from dental remains.Electrophoresis · 2019Article
- A method for extracting DNA from hard tissues for use in forensic identification.Biomedical reports · 2018Article
- Microbial Degradation of Forensic Samples of Biological Origin: Potential Threat to Human DNA Typing.Molecular biotechnology · 2018Review
- As solid as a rock-comparison of CE- and MPS-based analyses of the petrosal bone as a source of DNA for forensic identification of challenging cranial bones.International journal of legal medicine · 2018Article
- Tissue Microarray Analysis Applied to Bone Diagenesis.Scientific reports · 2017Article
- The auditory ossicles as a DNA source for genetic identification of highly putrefied cadavers.International journal of legal medicine · 2015Article
- Evaluation of carrier RNA and low volume demineralization for recovery of nuclear DNA from human teeth.Forensic science, medicine, and pathology · 2014Article
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3 authors.
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Abstract
There is a necessity for deceased identification as a result of many accidents and sometimes bones are the only accessible source of DNA. So far, a universal method that allows for extraction of DNA from materials at different stages of degradation does not exist. The aims of this study were: the comparison of three methods of DNA extraction from bones with different degree of degradation and an evaluation of the usefulness of these methods in forensic genetics. The efficiency of DNA extraction, the degree of extract contamination by polymerase chain reaction (PCR) inhibitors and the possibility of determining the STR loci profile were especially being compared. Nuclear DNA from bones at different states of degradation was isolated using three methods: classical, organic phenol-chloroform extraction, DNA extraction from crystal aggregates and extraction by total demineralisation. Total demineralisation is the best method for most cases of DNA extraction from bones, although it does not provide pure DNA. DNA extraction from aggregates removes inhibitors much better and is also a good method of choice when identity determination of exhumed remains is necessary. In the case of not buried bones (remains found outside) total demineralisation or phenol-chloroform protocols are more efficient for successful DNA extraction.
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