ArticleProceedings of the National Academy of Sciences of the United States of America2011
Unravelling evolutionary strategies of yeast for improving galactose utilization through integrated systems level analysis.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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58 citing papers in PubMed, 161 citations in OpenAlex.
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- Inducible chromosomal rearrangement reveals nonlinear polygenic dosage effects in driving aneuploid yeast traits.Nature communications · 2025Article
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- Resource presentation dictates genetic and phenotypic adaptation in yeast.BMC ecology and evolution · 2025Article
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- FromJournal of fungi (Basel, Switzerland) · 2023Review
- Recent progress in adaptive laboratory evolution of industrial microorganisms.Journal of industrial microbiology & biotechnology · 2023Article
- Past, Present, and Future Perspectives on Whey as a Promising Feedstock for Bioethanol Production by Yeast.Journal of fungi (Basel, Switzerland) · 2022Review
- Whole-cell modeling in yeast predicts compartment-specific proteome constraints that drive metabolic strategies.Nature communications · 2022Article
- Bioethanol production from defatted biomass of Nannochloropsis oculata microalgae grown under mixotrophic conditions.Environmental science and pollution research international · 2022Article
- Better under stress: Improving bacterial cellulose production byFrontiers in microbiology · 2022Article
- Development and characterization of efficient xylose utilization strains of Zymomonas mobilis.Biotechnology for biofuels · 2021Article
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4 authors at 1 institution in 3 countries.
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Abstract
Identification of the underlying molecular mechanisms for a derived phenotype by adaptive evolution is difficult. Here, we performed a systems-level inquiry into the metabolic changes occurring in the yeast Saccharomyces cerevisiae as a result of its adaptive evolution to increase its specific growth rate on galactose and related these changes to the acquired phenotypic properties. Three evolved mutants (62A, 62B, and 62C) with higher specific growth rates and faster specific galactose uptake were isolated. The evolved mutants were compared with a reference strain and two engineered strains, SO16 and PGM2, which also showed higher galactose uptake rate in previous studies. The profile of intermediates in galactose metabolism was similar in evolved and engineered mutants, whereas reserve carbohydrates metabolism was specifically elevated in the evolved mutants and one evolved strain showed changes in ergosterol biosynthesis. Mutations were identified in proteins involved in the global carbon sensing Ras/PKA pathway, which is known to regulate the reserve carbohydrates metabolism. We evaluated one of the identified mutations, RAS2(Tyr112), and this mutation resulted in an increased specific growth rate on galactose. These results show that adaptive evolution results in the utilization of unpredicted routes to accommodate increased galactose flux in contrast to rationally engineered strains. Our study demonstrates that adaptive evolution represents a valuable alternative to rational design in bioengineering of improved strains and, that through systems biology, it is possible to identify mutations in evolved strain that can serve as unforeseen metabolic engineering targets for improving microbial strains for production of biofuels and chemicals.
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