ArticleNucleic acids research2011
Transfection of plant mitochondria and in organello gene integration.
Article in Nucleic acids research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 46 citations in OpenAlex.
- Harnessing mitochondrial genome editing for crop improvement: principles and applications.aBIOTECH · 2026Review
- Mitochondrial genome of African rice (Mitochondrial DNA. Part B, Resources · 2026Article
- Tric proteins and TOM complex subunits are involved in the import of short DNA fragments into Arabidopsis mitochondria.Protoplasma · 2025Article
- The fate of artificial transgenes in Acanthamoeba castellanii.BMC genomics · 2025Article
- The Complete Mitochondrial Genome ofBiomolecules · 2025Article
- Three Parts of the Plant Genome: On the Way to Success in the Production of Recombinant Proteins.Plants (Basel, Switzerland) · 2022Review
- Polymer-coated carbon nanotube hybrids with functional peptides for gene delivery into plant mitochondria.Nature communications · 2022Article
- DNA lesions and repair in trypanosomatids infection.Genetics and molecular biology · 2020Article
- Revisiting trends on mitochondrial mega-channels for the import of proteins and nucleic acids.Journal of bioenergetics and biomembranes · 2017Review
- The Mosaic Mutants of Cucumber: A Method to Produce Knock-Downs of Mitochondrial Transcripts.G3 (Bethesda, Md.) · 2015Article
- The maintenance of mitochondrial DNA integrity--critical analysis and update.Cold Spring Harbor perspectives in biology · 2013Review
- Next-generation sequencing of mitochondrial targeted AAV transfer of human ND4 in mice.Molecular vision · 2013Article
- Gene delivery to mitochondria by targeting modified adenoassociated virus suppresses Leber's hereditary optic neuropathy in a mouse model.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- DNA delivery to mitochondria: sequence specificity and energy enhancement.Pharmaceutical research · 2011Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Investigation and manipulation of mitochondrial genetics in animal and plant cells remains restricted by the lack of an efficient in vivo transformation methodology. Mitochondrial transfection in whole cells and maintenance of the transfected DNA are main issues on this track. We showed earlier that isolated mitochondria from different organisms can import DNA. Exploiting this mechanism, we assessed the possibility to maintain exogenous DNA in plant organelles. Whereas homologous recombination is scarce in the higher plant nuclear compartment, recombination between large repeats generates the multipartite structure of the plant mitochondrial genome. These processes are under strict surveillance to avoid extensive genomic rearrangements. Nevertheless, following transfection of isolated organelles with constructs composed of a partial gfp gene flanked by fragments of mitochondrial DNA, we demonstrated in organello homologous recombination of the imported DNA with the resident DNA and integration of the reporter gene. Recombination yielded insertion of a continuous exogenous DNA fragment including the gfp sequence and at least 0.5 kb of flanking sequence on each side. According to our observations, transfection constructs carrying multiple sequences homologous to the mitochondrial DNA should be suitable and targeting of most regions in the organelle genome should be feasible, making the approach of general interest.
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