ArticleVirology journal2011
Real-world comparison of two molecular methods for detection of respiratory viruses.
Article in Virology journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 35 citations in OpenAlex.
- Viral Detection and Clinical Disease Features in Pediatric Chronic Rhinosinusitis.Laryngoscope investigative otolaryngology · 2026Article
- Epidemiology of HMPV and Other Respiratory Viral Infections Among Outpatients, 2016-2022.Open forum infectious diseases · 2026Article
- Respiratory viral infection is associated with increasedERJ open research · 2026Article
- Molecular characterization of human rhinovirus strains detected in pediatric acute respiratory tract infections.Iranian journal of microbiology · 2026Article
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- Assessing the Impact of Acute Respiratory Illnesses on the Risk of Subsequent Respiratory Illness.The Journal of infectious diseases · 2022Article
- The Impact of RSV-Associated Respiratory Disease on Children in Asia.Journal of pediatric infectious diseases · 2019Review
- DETECTION OF NON-INFLUENZA VIRUSES IN ACUTE RESPIRATORY INFECTIONS IN CHILDREN UNDER FIVE-YEAR-OLD IN COTE D'IVOIRE (JANUARY - DECEMBER 2013).African journal of infectious diseases · 2018Article
- Dual RNA-seq reveals viral infections in asthmatic children without respiratory illness which are associated with changes in the airway transcriptome.Genome biology · 2017Article
- Article
- Respiratory Viral Detections During Symptomatic and Asymptomatic Periods in Young Andean Children.The Pediatric infectious disease journal · 2015Article
- Detection of respiratory viruses in gargle specimens of healthy children.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2015Article
- Human rhinovirus C infections in pediatric hematology and oncology patients.Pediatric transplantation · 2015Article
- A household-based study of acute viral respiratory illnesses in Andean children.The Pediatric infectious disease journal · 2014Article
- Viral etiologies of infant bronchiolitis, croup and upper respiratory illness during 4 consecutive years.The Pediatric infectious disease journal · 2013Article
- Molecular detection of human metapneumovirus and human bocavirus on oropharyngeal swabs collected from young children with acute respiratory tract infections from rural and peri-urban communities in South India.Molecular diagnosis & therapy · 2013Article
- New molecular virus detection methods and their clinical value in lower respiratory tract infections in children.Paediatric respiratory reviews · 2013Review
- HexaPrime: a novel method for detection of coronaviruses.Journal of virological methods · 2013Article
- Burden of human metapneumovirus infection in young children.The New England journal of medicine · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundMolecular polymerase chain reaction (PCR) based assays are increasingly used to diagnose viral respiratory infections and conduct epidemiology studies. Molecular assays have generally been evaluated by comparing them to conventional direct fluorescent antibody (DFA) or viral culture techniques, with few published direct comparisons between molecular methods or between institutions. We sought to perform a real-world comparison of two molecular respiratory viral diagnostic methods between two experienced respiratory virus research laboratories.
methodsWe tested nasal and throat swab specimens obtained from 225 infants with respiratory illness for 11 common respiratory viruses using both a multiplex assay (Respiratory MultiCode-PLx Assay [RMA]) and individual real-time RT-PCR (RT-rtPCR).
resultsBoth assays detected viruses in more than 70% of specimens, but there was discordance. The RMA assay detected significantly more human metapneumovirus (HMPV) and respiratory syncytial virus (RSV), while RT-rtPCR detected significantly more influenza A. We speculated that primer differences accounted for these discrepancies and redesigned the primers and probes for influenza A in the RMA assay, and for HMPV and RSV in the RT-rtPCR assay. The tests were then repeated and again compared. The new primers led to improved detection of HMPV and RSV by RT-rtPCR assay, but the RMA assay remained similar in terms of influenza detection.
conclusionsGiven the absence of a gold standard, clinical and research laboratories should regularly correlate the results of molecular assays with other PCR based assays, other laboratories, and with standard virologic methods to ensure consistency and accuracy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.