ArticleAnalytical and bioanalytical chemistry2011
Analysis of urinary estrogens, their oxidized metabolites, and other endogenous steroids by benchtop orbitrap LCMS versus traditional quadrupole GCMS.
Article in Analytical and bioanalytical chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.
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Who cites it
23 citing papers in PubMed, 1 synthesis or guideline pooled it, 44 citations in OpenAlex.
- Endogenous Circulating Sex Hormone Concentrations and Colon Cancer Risk in Postmenopausal Women: A Prospective Study and Meta-Analysis.JNCI cancer spectrum · 2021Pooled it
- Dexamethasone facilitates fear extinction and safety discrimination in PTSD: A placebo-controlled, double-blind study.Psychoneuroendocrinology · 2017Trial
- Soy Food Intake and Biomarkers of Breast Cancer Risk: Possible Difference in Asian Women?Nutrition and cancer · 2017Trial
- Oestrogen levels in serum and urine of premenopausal women eating low and high amounts of meat.Public health nutrition · 2014Trial
- The relation of urinary estrogen metabolites with mammographic densities in premenopausal women.Cancer epidemiology · 2012Trial
- Urinary estrogen metabolites in two soy trials with premenopausal women.European journal of clinical nutrition · 2012Trial
- A Membrane-Based Strategy for the High-Throughput Determination of Steroidal Hormones in Human Urine Using Natural Deep Eutectic Solvents Combined With Liquid Chromatography Coupled With Diode Array Detector.Journal of separation science · 2025Article
- Urinary estrogen metabolites and breast cancer risk in Chinese population.Endocrine connections · 2021Article
- Urinary oestrogen steroidome as an indicator of the risk of localised prostate cancer progression.British journal of cancer · 2021Article
- Epigenetic prediction of 17β-estradiol and relationship to trauma-related outcomes in women.Comprehensive psychoneuroendocrinology · 2021Article
- Simultaneous detection of diethylstilbestrol and estradiol residues with a single immunochromatographic assay strip.Food science & nutrition · 2021Article
- Current strategies for quantification of estrogens in clinical research.The Journal of steroid biochemistry and molecular biology · 2019Review
- Derivatization enhances analysis of estrogens and their bioactive metabolites in human plasma by liquid chromatography tandem mass spectrometry.Analytica chimica acta · 2019Article
- Data for analysis of catechol estrogen metabolites in human plasma by liquid chromatography tandem mass spectrometry.Data in brief · 2019Article
- Trends in the application of high-resolution mass spectrometry for human biomonitoring: An analytical primer to studying the environmental chemical space of the human exposome.Environment international · 2017Review
- β-Estradiol and ethinyl-estradiol contamination in the rivers of the Carpathian Basin.Environmental science and pollution research international · 2016Article
- Analysis of estrogens and androgens in postmenopausal serum and plasma by liquid chromatography-mass spectrometry.Steroids · 2015Review
- Article
- Improvement of bisphenol A quantitation from urine by LCMS.Analytical and bioanalytical chemistry · 2015Article
- Association of estrogen measurements in serum and urine of premenopausal women.Biomarkers in medicine · 2015Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
Estrogens and other endogenous steroids are known risk markers for cancer. Gas chromatography (GC) with mass spectrometry (MS) has traditionally predominated the analysis of estrogens and other endogenous steroids, but liquid chromatography (LC) MS is increasingly favored. Direct comparisons of the two technologies have hitherto not been performed. Steroids were analyzed from 232 urine samples of 78 premenopausal women in a blinded fashion by benchtop orbitrap LCMS and single quadrupole GCMS. Sixteen steroidal estrogens including oxidized metabolites could be analyzed by LCMS. LCMS-GCMS Spearman rank correlations of the major estrogens E(1), E(2), E(3), 16α-OHE(1), and 2-OHE(1) were very high (r = 0.72-0.91), and absolute concentrations also agreed (<5% difference for E(1), E(2), E(3), 16α-OHE(1)). LCMS allowed reinterrogation of the acquired data due to orbitrap technology, which permitted post-analysis quantitation of progesterone, cortisol, and cortisone (LCMS-GCMS Spearman rank correlations = 0.80-0.84; absolute difference, <7%; n = 137). GCMS allows the measurement of a wide range of steroids including non-polar analytes that escape the presented LCMS assay. In contrast, orbitrap-based LCMS can detect more estrogens, is faster, less costly, allows post-data acquisition reinterrogation of certain analytes that had not been targeted a priori, and requires much less urine.
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Registered trials
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