ArticleArthritis research & therapy2011
Identification of target antigens of anti-endothelial cell and anti-vascular smooth muscle cell antibodies in patients with giant cell arteritis: a proteomic approach.
Article in Arthritis research & therapy, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 51 citations in OpenAlex.
- Anti-endothelial cell antibodies in pathogenesis of vasculitis.Frontiers in immunology · 2025Review
- Review
- The innate face of Giant Cell Arteritis: Insight into cellular and molecular innate immunity pathways to unravel new possible biomarkers of disease.Frontiers in molecular medicine · 2022Review
- A Master Autoantigen-ome Links Alternative Splicing, Female Predilection, and COVID-19 to Autoimmune Diseases.bioRxiv : the preprint server for biology · 2021Article
- Mechanism and biomarkers in aortitis--a review.Journal of molecular medicine (Berlin, Germany) · 2020Review
- Massive B-Cell Infiltration and Organization Into Artery Tertiary Lymphoid Organs in the Aorta of Large Vessel Giant Cell Arteritis.Frontiers in immunology · 2019Article
- [New biomarkers for large vessel vasculitis and spondyloarthropathies].Zeitschrift fur Rheumatologie · 2017Article
- Antigenicity of Bovine Pericardium Determined by a Novel Immunoproteomic Approach.Scientific reports · 2017Article
- Giant Cell Arteritis: From Pathogenesis to Therapeutic Management.Current treatment options in rheumatology · 2016Article
- Inflammatory neuropathies: pathology, molecular markers and targets for specific therapeutic intervention.Acta neuropathologica · 2015Review
- Parietal and intravascular innate mechanisms of vascular inflammation.Arthritis research & therapy · 2015Review
- An Optimized Fluorescence-Based Bidimensional Immunoproteomic Approach for Accurate Screening of Autoantibodies.PloS one · 2015Article
- Identification of 4-Trimethylaminobutyraldehyde Dehydrogenase (TMABA-DH) as a Candidate Serum Autoantibody Target for Kawasaki Disease.PloS one · 2015Article
- Neurotrophins are expressed in giant cell arteritis lesions and may contribute to vascular remodeling.Arthritis research & therapy · 2014Article
- Dietary vitamin D deficiency in rats from middle to old age leads to elevated tyrosine nitration and proteomics changes in levels of key proteins in brain: implications for low vitamin D-dependent age-related cognitive decline.Free radical biology & medicine · 2013Article
- Immune mechanisms in medium and large-vessel vasculitis.Nature reviews. Rheumatology · 2013Review
- Interaction of aldehydes derived from lipid peroxidation and membrane proteins.Frontiers in physiology · 2013Review
- The immunopathology of giant cell arteritis: diagnostic and therapeutic implications.Journal of neuro-ophthalmology : the official journal of the North American Neuro-Ophthalmology Society · 2012Review
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Authors and funding
11 authors at 6 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionImmunological studies of giant cell arteritis (GCA) suggest that a triggering antigen of unknown nature could generate a specific immune response. We thus decided to detect autoantibodies directed against endothelial cells (ECs) and vascular smooth muscle cells (VSMCs) in the serum of GCA patients and to identify their target antigens.
methodsSera from 15 GCA patients were tested in 5 pools of 3 patients' sera and compared to a sera pool from 12 healthy controls (HCs). Serum immunoglobulin G (IgG) reactivity was analysed by 2-D electrophoresis and immunoblotting with antigens from human umbilical vein ECs (HUVECs) and mammary artery VSMCs. Target antigens were identified by mass spectrometry.
resultsSerum IgG from GCA patients recognised 162 ± 3 (mean ± SD) and 100 ± 17 (mean ± SD) protein spots from HUVECs and VSMCs, respectively, and that from HCs recognised 79 and 94 protein spots, respectively. In total, 30 spots from HUVECs and 19 from VSMCs were recognised by at least two-thirds and three-fifths, respectively, of the pools of sera from GCA patients and not by sera from HCs. Among identified proteins, we found vinculin, lamin A/C, voltage-dependent anion-selective channel protein 2, annexin V and other proteins involved in cell energy metabolism and key cellular pathways. Ingenuity pathway analysis revealed that most identified target antigens interacted with growth factor receptor-bound protein 2.
conclusionsIgG antibodies to proteins in the proteome of ECs and VSMCs are present in the sera of GCA patients and recognise cellular targets that play key roles in cell biology and maintenance of homeostasis. Their potential pathogenic role remains to be determined.
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