ArticleAnalytical chemistry2011
Anti-DNA:RNA antibodies and silicon photonic microring resonators: increased sensitivity for multiplexed microRNA detection.
Article in Analytical chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 159 citations in OpenAlex.
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- Rapid detection of an Ebola biomarker with optical microring resonators.Cell reports methods · 2022Article
- Multiplex optical biosensors based on multi-pinhole interferometry.Biomedical optics express · 2021Article
- Recent trends in application of nanomaterials for the development of electrochemical microRNA biosensors.Mikrochimica acta · 2021Review
- Photonic technologies for liquid biopsies: recent advances and open research challenges.Laser & photonics reviews · 2021Article
- Microfluidic Packaging Integration with Electronic-Photonic Biosensors Using 3D Printed Transfer Molding.Biosensors · 2020Article
- Optical Biosensors for the Detection of Rheumatoid Arthritis (RA) Biomarkers: A Comprehensive Review.Sensors (Basel, Switzerland) · 2020Review
- Whispering-Gallery Sensors.Matter · 2020Review
- Simultaneous and ultrasensitive detection of multiple microRNAs by single-molecule fluorescence imaging.Chemical science · 2020Article
- Manipulating the hydrophobicity of DNA as a universal strategy for visual biosensing.Nature protocols · 2020Article
- Label-Free MicroRNA Optical Biosensors.Nanomaterials (Basel, Switzerland) · 2019Review
- Optical Biosensors Based on Silicon-On-Insulator Ring Resonators: A Review.Molecules (Basel, Switzerland) · 2019Review
- Technological Challenges and Future Issues for the Detection of Circulating MicroRNAs in Patients With Cancer.Frontiers in chemistry · 2019Review
- Multiplexed microRNA Expression Profiling by Combined Asymmetric PCR and Label-Free Detection using Silicon Photonic Sensor Arrays.Analytical methods : advancing methods and applications · 2018Article
- High sensitivity and label-free oligonucleotides detection using photonic bandgap sensing structures biofunctionalized with molecular beacon probes.Biomedical optics express · 2018Article
- Experimental Study of the Oriented Immobilization of Antibodies on Photonic Sensing Structures by Using Protein A as an Intermediate Layer.Sensors (Basel, Switzerland) · 2018Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
In this paper, we present a method for the sensitive detection of microRNAs (miRNAs) utilizing an antibody that specifically recognizes DNA:RNA heteroduplexes and a silicon photonic microring resonator array transduction platform. Microring resonator arrays are covalently functionalized with DNA capture probes that are complementary to solution phase miRNA targets. Following hybridization on the sensor, the anti-DNA:RNA antibody is introduced and binds selectively to the heteroduplexes, giving a larger signal than the original miRNA hybridization due to the increased mass of the antibody, as compared to the 22-mer oligoribonucleotide. Furthermore, the secondary recognition step is performed in neat buffer solution and at relatively higher antibody concentrations, facilitating the detection of miRNAs of interest. The intrinsic sensitivity of the microring resonator platform coupled with the amplification provided by the anti-DNA:RNA antibodies allows for the detection of microRNAs at concentrations as low as 10 pM (350 amol). The simplicity and sequence generality of this amplification method position it as a promising tool for high-throughput, multiplexed miRNA analysis as well as a range of other RNA based detection applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.