ArticleProceedings of the National Academy of Sciences of the United States of America2011
Chemotactic cell trapping in controlled alternating gradient fields.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 78 citations in OpenAlex.
- Quantitative, Label-Free Mapping of Cell Force Dynamics.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Traveling-wave chemotaxis of neutrophil-like HL-60 cells.Molecular biology of the cell · 2025Article
- Cross-Species Applications of Peptide Substrate Reporters to Quantitative Measurements of Kinase Activity.ACS measurement science au · 2024Article
- The MAPK/ERK channel capacity exceeds 6 bit/hour.PLoS computational biology · 2023Article
- Short-time diffusive fluxes over membrane receptors yields the direction of a signalling source.Royal Society open science · 2023Article
- On-demand microfluidic mixing by actuating integrated magnetic microwalls.Lab on a chip · 2023Article
- Coupling cell shape and velocity leads to oscillation and circling in keratocyte galvanotaxis.Biophysical journal · 2023Article
- Covalent transfer of chemical gradients onto a graphenic surface with 2D and 3D control.Nature communications · 2022Article
- Understanding the Cell's Response to Chemical Signals: Utilisation of Microfluidic Technology in Studies of Cellular andMicromachines · 2022Review
- Generation of Dynamic Concentration Profile Using A Microfluidic Device Integrating Pneumatic Microvalves.Biosensors · 2022Article
- Critical role of lipid membranes in polarization and migration of cells: a biophysical view.Biophysical reviews · 2021Review
- Bridging from single to collective cell migration: A review of models and links to experiments.PLoS computational biology · 2020Review
- Heterogeneities Shape Passive Intracellular Transport.Biophysical journal · 2019Article
- Screening by changes in stereotypical behavior during cell motility.Scientific reports · 2019Article
- Diffusing wave paradox of phototactic particles in traveling light pulses.Nature communications · 2019Article
- Hydrodynamics in Cell Studies.Chemical reviews · 2018Review
- Acoustic Actuation ofJournal of micromechanics and microengineering : structures, devices, and systems · 2018Article
- Simultaneous or Sequential Orthogonal Gradient Formation in a 3D Cell Culture Microfluidic Platform.Small (Weinheim an der Bergstrasse, Germany) · 2016Article
- A spatiotemporally controllable chemical gradient generator via acoustically oscillating sharp-edge structures.Lab on a chip · 2015Article
- Acoustofluidic chemical waveform generator and switch.Analytical chemistry · 2014Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Directed cell migration toward spatio-temporally varying chemotactic stimuli requires rapid cytoskeletal reorganization. Numerous studies provide evidence that actin reorganization is controlled by intracellular redistribution of signaling molecules, such as the PI4,5P2/PI3,4,5P3 gradient. However, exploring underlying mechanisms is difficult and requires careful spatio-temporal control of external chemotactic stimuli. We designed a microfluidic setup to generate alternating chemotactic gradient fields for simultaneous multicell exposure, greatly facilitating statistical analysis. For a quantitative description of intracellular response dynamics, we apply alternating time sequences of spatially homogeneous concentration gradients across 300 μm, reorienting on timescales down to a few seconds. Dictyostelium discoideum amoebae respond to gradient switching rates below 0.02 Hz by readapting their migration direction. For faster switching, cellular repolarization ceases and is completely stalled at 0.1 Hz. In this "chemotactically trapped" cell state, external stimuli alternate faster than intracellular feedback is capable to respond by onset of directed migration. To investigate intracellular actin cortex rearrangement during gradient switching, we correlate migratory cell response with actin repolymerization dynamics, quantified by a fluorescence distribution moment of the GFP fusion protein LimEΔcc. We find two fundamentally different cell polarization types and we could reveal the role of PI3-Kinase for cellular repolarization. In the early aggregation phase, PI3-Kinase enhances the capability of D. discoideum cells to readjust their polarity in response to spatially alternating gradient fields, whereas in aggregation competent cells the effect of PI3-Kinase perturbation becomes less relevant.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.