ArticleBMC microbiology2011
A multiplex real-time PCR assay targeting virulence and resistance genes in Salmonella enterica serotype Typhimurium.
Article in BMC microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 36 citations in OpenAlex.
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- "Does the Salmonella Genomic Island 1 (SGI1) confer invasiveness properties to human isolates?"BMC infectious diseases · 2017Article
- WGS accurately predicts antimicrobial resistance in Escherichia coli.The Journal of antimicrobial chemotherapy · 2015Article
- Bovine salmonellosis in northeast of Iran: frequency, genetic fingerprinting and antimicrobial resistance patterns of Salmonella spp.Asian Pacific journal of tropical biomedicine · 2014Article
- Phenotypic-genotypic resistance in Salmonella spp. isolated from cattle carcasses from the north central zone of the State of Mexico.Tropical animal health and production · 2013Article
- Identification of immunogenic proteins and generation of antibodies against Salmonella Typhimurium using phage display.BMC biotechnology · 2012Article
- Detection of Shiga toxin-producing Escherichia coli in ground beef using the GeneDisc real-time PCR system.Frontiers in cellular and infection microbiology · 2012Article
- ArmA methyltransferase in a monophasic Salmonella enterica isolate from food.Antimicrobial agents and chemotherapy · 2011Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTyphimurium is the main serotype of Salmonella enterica subsp. enterica implicated in food-borne diseases worldwide. This study aimed to detect the prevalence of ten markers combined in a macro-array based on multiplex real-time PCR. We targeted characteristic determinants located on pathogenicity islands (SPI-2 to -5, virulence plasmid pSLT and Salmonella genomic island 1 (SGI1)) as well as a specific 16S-23S rRNA intergenic spacer sequence of definitive type 104 (DT104). To investigate antimicrobial resistance, the study also targeted the presence of genes involved in sulfonamide (sul1) and beta-lactam (blaTEM) resistance. Finally, the intI1 determinant encoding integrase from class 1 integron was also investigated.
resultsA total of 538 unrelated S. Typhimurium strains isolated between 1999 and 2009 from various sources, including food animals, food products, human and environmental samples were studied. Based on the combined presence or absence of these markers, we distinguished 34 different genotypes, including three major genotypes encountered in 75% of the studied strains, Although SPI determinants were almost always detected, SGI1, intI1, sul1 and blaTEM determinants were found 47%, 52%, 54% and 12% of the time respectively, varying according to isolation source. Low-marker patterns were most often detected in poultry sources whereas full-marker patterns were observed in pig, cattle and human sources.
conclusionThe GeneDisc® assay developed in this study madeit easier to explore variability within serotype Typhimurium by analyzing ten relevant gene determinants in a large collection of strains. This real-time multiplex method constitutes a valuable tool for strains characterization on epidemiological purposes.
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