ArticleCellular microbiology2011
In search of Brucella abortus type IV secretion substrates: screening and identification of four proteins translocated into host cells through VirB system.
Article in Cellular microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
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Who cites it
70 citing papers in PubMed.
- Article
- A subset of type 4 secretion system effectors ofmicroLife · 2026Article
- ThemicroLife · 2025Review
- Identification of Brucella RS15060 as a novel type IV secretion system effector associated with bacterial virulence.Veterinary research · 2024Article
- Copper sensing transcription factor ArsR2 regulates VjbR to sustain virulence inEmerging microbes & infections · 2024Article
- Article
- Article
- Antibiotic persistence of intracellular Brucella abortus.PLoS neglected tropical diseases · 2022Article
- Article
- Virulence factors perforate the pathogen-containing vacuole to signal efferocytosis.Cell host & microbe · 2022Article
- Biochemical and functional characterization ofFrontiers in microbiology · 2022Article
- Seroepidemiology of Camel Brucellosis in and around Dire Dawa, Eastern Ethiopia.Veterinary medicine international · 2022Article
- A Brucella effector modulates the Arf6-Rab8a GTPase cascade to promote intravacuolar replication.The EMBO journal · 2021Article
- TheProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Uncovering the Hidden Credentials ofMicrobiology and molecular biology reviews : MMBR · 2021Review
- Computational prediction of secreted proteins in gram-negative bacteria.Computational and structural biotechnology journal · 2021Review
- Structural Profiling of Bacterial Effectors Reveals Enrichment of Host-Interacting Domains and Motifs.Frontiers in molecular biosciences · 2021Article
- Proteomics of Brucella.Proteomes · 2020Review
- Epistatic Interplay between Type IV Secretion Effectors Engages the Small GTPase Rab2 in themBio · 2020Article
- Article
10 more citing papers are in PubMed but not listed here.
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5 authors.
Funding
Abstract
Type IV secretion systems (T4SS) are specialized protein complexes used by many bacterial pathogens for the delivery of effector molecules that subvert varied host cellular processes. Brucella spp. are facultative intracellular pathogens capable of survival and replication inside mammalian cells. Brucella T4SS (VirB) is essential to subvert lysosome fusion and to create an organelle permissive for replication. One possible role for VirB is to translocate effector proteins that modulate host cellular functions for the biogenesis of the replicative organelle. We hypothesized that proteins with eukaryotic domains or protein-protein interaction domains, among others, would be good candidates for modulation of host cell functions. To identify these candidates, we performed an in silico screen looking for proteins with distinctive features. Translocation of 84 potential substrates was assayed using adenylate cyclase reporter. By this approach, we identified six proteins that are delivered to the eukaryotic cytoplasm upon infection of macrophage-like cells and we could determine that four of them, encoded by genes BAB1_1043, BAB1_2005, BAB1_1275 and BAB2_0123, require a functional T4SS for their delivery. We confirmed VirB-mediated translocation of one of the substrates by immunofluorescence confocal microscopy, and we found that the N-terminal 25 amino acids are required for its delivery into cells.
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