ArticleFEMS microbiology letters2011
Genetic transformation of Veillonella parvula.
Article in FEMS microbiology letters, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed, 15 citations in OpenAlex.
- Establishment of a HicA toxin-based counterselection system for markerless genetic engineering in Veillonella atypica OK5.BMC microbiology · 2026Article
- Complete genome sequence ofMicrobiology resource announcements · 2025Article
- Development of a small shuttle plasmid for use in oral Veillonella and initial appraisal of potential for fluorescence-based applications.Letters in applied microbiology · 2024Article
- Illuminating the oral microbiome and its host interactions: tools and approaches for molecular microbiology studies.FEMS microbiology reviews · 2023Review
- Case Report: Oral and fecal microbiota in a super-donor: the healthy microbiota paradigm for fecal transplantation.Frontiers in microbiomes · 2023Article
- Characterization of Intestinal Microbiota in Lambs with Different Susceptibility toVeterinary sciences · 2022Article
- Veillonellae: Beyond Bridging Species in Oral Biofilm Ecology.Frontiers in oral health · 2021Review
- [Research progress in the relationship between Veillonella and oral diseases].Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology · 2020Review
- Veillonella Catalase Protects the Growth of Fusobacterium nucleatum in Microaerophilic and Streptococcus gordonii-Resident Environments.Applied and environmental microbiology · 2017Article
- Complete Genome Sequence ofGenome announcements · 2017Article
- Article
- Natural Competence Is Common among Clinical Isolates ofFrontiers in cellular and infection microbiology · 2017Article
- Establishment of a tractable genetic transformation system in Veillonella spp.Applied and environmental microbiology · 2012Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Veillonellae are one of the most prevalent and predominant microorganisms in both the supra- and subgingival plaques of the human oral cavity. Veillonellae's mutualistic relationships with the early, middle, and late colonizers of the oral cavity make them an important component of oral biofilm ecology. Unlike other ubiquitous early colonizers in the oral cavity, surprisingly little is known about Veillonella biology due to our lack of ability to genetically transform this group of bacteria. The objective of this study was to test the transformability of veillonellae. Using Veillonella parvula strain PK1910, we first obtained spontaneous mutations conferring streptomycin resistance. These mutations all carry a K43N substitution in the RpsL protein. Using the mutated rpsL gene as a selection marker, a variety of conditions were tested and optimized for electroporation. With the optimized protocol, we were able to introduce the first targeted mutation into the chromosome of V. parvula PK1910. Although more studies are needed to develop a robust genetic manipulation system in veillonellae, our results demonstrated, for the first time, that V. parvula is transformable, at least for strain PK1910.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.