ArticleTumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine2011
Plasma membrane proteomic analysis of human osteosarcoma and osteoblastic cells: revealing NDRG1 as a marker for osteosarcoma.
Article in Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 36 citations in OpenAlex.
- CCCTC-binding factor regulates splicing factor proline and glutamine-rich to promote malignant growth of osteosarcoma.American journal of translational research · 2025Article
- Article
- NDRG1 in Cancer: A Suppressor, Promoter, or Both?Cancers · 2022Review
- Mass Spectrometric-Based Proteomics for Biomarker Discovery in Osteosarcoma: Current Status and Future Direction.International journal of molecular sciences · 2022Review
- Biological evidence of cancer stem-like cells and recurrent disease in osteosarcoma.Cancer drug resistance (Alhambra, Calif.) · 2022Review
- Article
- KPNA2 expression is a potential marker for differential diagnosis between osteosarcomas and other malignant bone tumor mimics.Diagnostic pathology · 2020Article
- Circular RNA CircITGA7 Promotes Tumorigenesis of Osteosarcoma via miR-370/PIM1 Axis.Computational and mathematical methods in medicine · 2020Article
- NDRG1 inhibition sensitizes osteosarcoma cells to combretastatin A-4 through targeting autophagy.Cell death & disease · 2017Article
- Comparative proteomic investigation of metastatic and non-metastatic osteosarcoma cells of human and canine origin.PloS one · 2017Article
- Exploring targeted therapy of osteosarcoma using proteomics data.OncoTargets and therapy · 2017Article
- SPAG9 is overexpressed in osteosarcoma, and regulates cell proliferation and invasion through regulation of JunD.Oncology letters · 2016Article
- Biomarkers for Bone Tumors: Discovery from Genomics and Proteomics Studies and Their Challenges.Molecular medicine (Cambridge, Mass.) · 2016Article
- Cancer cell-specific oligopeptides selected by an integrated microfluidic system from a phage display library for ovarian cancer diagnosis.Theranostics · 2015Article
- CIP2A is overexpressed in osteosarcoma and regulates cell proliferation and invasion.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2014Article
- Surface proteomic analysis of osteosarcoma identifies EPHA2 as receptor for targeted drug delivery.British journal of cancer · 2013Article
- Comparative analysis of the surface exposed proteome of two canine osteosarcoma cell lines and normal canine osteoblasts.BMC veterinary research · 2013Article
- Serum tumor markers in pediatric osteosarcoma: a summary review.Clinical sarcoma research · 2012Article
- Proteomic technologies for the study of osteosarcoma.Sarcoma · 2012Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Osteosarcoma (OS) is the most common primary malignant bone tumor in children and adolescents. To identify new biomarkers for early diagnosis of OS and novel therapeutic candidates, we carried out a plasma membrane proteomic study based on two-dimensional electrophoresis (2DE). The OS cell line MG-63 and the human osteoblastic cell line hFOB1.19 were adopted as the comparison model. We extracted plasma membrane by aqueous two-phase partition extraction. The proteins were separated through 2DE. We analyzed the differentially expressed proteins by Imagemaster software and then identified them by liquid chromatography-tandem mass spectrometry, and the location and function of differential proteins were searched through the Gene Ontology database. In total, 220 protein spots were separated by 2DE. Seven proteins with more than 2.0-folds of difference were successfully identified from 13 gel spots, with 6 up-regulated and 1 down-regulated. Gene Ontology analysis of the differentially expressed proteins indicated that these proteins were involved in seven kinds of functions including binding, structural, cell motility, receptor activity, electron carrier activity, NADH dehydrogenase (ubiquinone) activity, and transcription repressor activity. The up-regulation of NDRG1 was verified in osteosarcoma through Western blotting and by immunohistochemistry in paraffin-embedded tissues. The plasma membrane proteins identified in this study may provide new insights into osteosarcoma cancer biology and potential diagnostic and therapeutic biomarkers.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.