ArticleApplied and environmental microbiology2011
Role of rpoS in the development of cell envelope resilience and pressure resistance in stationary-phase Escherichia coli.
Article in Applied and environmental microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 62 citations in OpenAlex.
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- Yersinia pseudotuberculosis doxycycline tolerance strategies include modulating expression of genes involved in cell permeability and tRNA modifications.PLoS pathogens · 2022Article
- Cyclopropane Fatty Acids Are Important forInfection and immunity · 2022Article
- Heat and Pressure Resistance inFrontiers in microbiology · 2020Article
- Transcriptional Sequencing Uncovers Survival Mechanisms of Salmonella enterica Serovar Enteritidis in Antibacterial Egg White.mSphere · 2019Article
- The role ofInfection and drug resistance · 2019Article
- Suppression of Alternative Lipooligosaccharide Glycosyltransferase Activity by UDP-Galactose Epimerase Enhances Murine Lung Infection and Evasion of Serum IgM.Frontiers in cellular and infection microbiology · 2019Article
- Functional analysis of Salmonella Typhi adaptation to survival in water.Environmental microbiology · 2018Article
- Interrelation between Tween and the membrane properties and high pressure tolerance of Lactobacillus plantarum.BMC microbiology · 2018Article
- Population-Wide Survey of Salmonella enterica Response to High-Pressure Processing Reveals a Diversity of Responses and Tolerance Mechanisms.Applied and environmental microbiology · 2018Article
- Stress-Adaptive Responses Associated with High-Level Carbapenem Resistance in KPC-ProducingJournal of pathogens · 2018Article
- RpoS-independent evolution reveals the importance of attenuated cAMP/CRP regulation in high hydrostatic pressure resistance acquisition in E. coli.Scientific reports · 2017Article
- Proteome remodelling by the stress sigma factor RpoS/σScientific reports · 2017Article
- Global Gene Expression Analysis of Cross-Protected Phenotype of Pectobacterium atrosepticum.PloS one · 2017Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This work investigated the role of rpoS in the development of increased cell envelope resilience and enhanced pressure resistance in stationary-phase cells of Escherichia coli. Loss of both colony-forming ability and membrane integrity, measured as uptake of propidium iodide (PI), occurred at lower pressures in E. coli BW3709 (rpoS) than in the parental strain (BW2952). The rpoS mutant also released much higher concentrations of protein under pressure than the parent. We propose that RpoS-regulated functions are responsible for the increase in membrane resilience as cells enter stationary phase and that this plays a major role in the development of pressure resistance. Strains from the Keio collection with mutations in two RpoS-regulated genes, cfa (cyclopropane fatty acyl phospholipid synthase) and osmB (outer membrane lipoprotein), were significantly more pressure sensitive and took up more PI than the parent strain, with cfa having the greatest effect. Mutations in the bolA morphogene and other RpoS-regulated lipoprotein genes (osmC, osmE, osmY, and ybaY) had no effect on pressure resistance. The cytoplasmic membranes of the rpoS mutant failed to reseal after pressure treatment, and strains with mutations in osmB and nlpI (new lipoprotein) were also somewhat impaired in the ability to reseal their membranes. The cfa mutant, though pressure sensitive, was unaffected in membrane resealing, implying that the initial transient permeabilization event is critical for loss of viability rather than the failure to reseal. The enhanced pressure sensitivity of polA, recA, and xthA mutants suggested that DNA may be a target of oxidative stress in pressure-treated cells.
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