Trial reportApplied and environmental microbiology2011
Selection of fecal enterococci exhibiting tcrB-mediated copper resistance in pigs fed diets supplemented with copper.
Trial report in Applied and environmental microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 70 citations in OpenAlex.
- Comparative Analysis of Two Consecutive Genome Sequencing Results ofArchives of Razi Institute · 2025Article
- A Critical Review of AMR Risks Arising as a Consequence of Using Biocides and Certain Metals in Food Animal Production.Antibiotics (Basel, Switzerland) · 2023Review
- Effect of high-copper diet on transference ofFrontiers in veterinary science · 2023Article
- Factors associated with antimicrobial resistant enterococci in Canadian beef cattle: A scoping review.Frontiers in veterinary science · 2023Article
- Role of horizontally transferred copper resistance genes inMicrobiology (Reading, England) · 2022Review
- Effect of Protracted Free-Choice Chlortetracycline-Medicated Mineral for Anaplasmosis Control onMicroorganisms · 2021Article
- Directed Recovery and Molecular Characterization of Antibiotic Resistance Plasmids from Cheese Bacteria.International journal of molecular sciences · 2021Article
- The Effects of Natural Products and Environmental Conditions on Antimicrobial Resistance.Molecules (Basel, Switzerland) · 2021Review
- Unravelling the antibiotic and heavy metal resistome of a chronically polluted soil.3 Biotech · 2020Article
- Effects of copper and zinc sources and inclusion levels of copper on weanling pig performance and intestinal microbiota.Journal of animal science · 2020Article
- Impact of added copper, alone or in combination with chlortetracycline, on growth performance and antimicrobial resistance of fecal enterococci of weaned piglets.Journal of animal science · 2020Article
- Resistance to change: AMR gene dynamics on a commercial pig farm with high antimicrobial usage.Scientific reports · 2020Article
- Effect of Dietary Copper on Intestinal Microbiota and Antimicrobial Resistance Profiles ofFrontiers in microbiology · 2019Article
- Selection and dissemination of antimicrobial resistance in Agri-food production.Antimicrobial resistance and infection control · 2019Review
- Distribution of theGenes · 2018Article
- Antimicrobial Resistance inMicrobiology spectrum · 2018Review
- Antimicrobial resistance of Enterococcus faecium strains isolated from commercial probiotic products used in cattle and swine.Journal of animal science · 2018Article
- Antimicrobial resistance due to the content of potentially toxic metals in soil and fertilizing products.Microbial ecology in health and disease · 2018Review
- Comparison of antibiotic resistance and copper tolerance of Enterococcus spp. and Lactobacillus spp. isolated from piglets before and after weaning.Journal of microbiology (Seoul, Korea) · 2017Article
- EMA and EFSA Joint Scientific Opinion on measures to reduce the need to use antimicrobial agents in animal husbandry in the European Union, and the resulting impacts on food safety (RONAFA).EFSA journal. European Food Safety Authority · 2017Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Copper, as copper sulfate, is increasingly used as an alternative to in-feed antibiotics for growth promotion in weaned piglets. Acquired copper resistance, conferred by a plasmid-borne, transferable copper resistance (tcrB) gene, has been reported in Enterococcus faecium and E. faecalis. A longitudinal field study was undertaken to determine the relationship between copper supplementation and the prevalence of tcrB-positive enterococci in piglets. The study was done with weaned piglets, housed in 10 pens with 6 piglets per pen, fed diets supplemented with a normal (16.5 ppm; control) or an elevated (125 ppm) level of copper. Fecal samples were randomly collected from three piglets per pen on days 0, 14, 28, and 42 and plated on M-Enterococcus agar, and three enterococcal isolates were obtained from each sample. The overall prevalence of tcrB-positive enterococci was 21.1% (38/180) in piglets fed elevated copper and 2.8% (5/180) in the control. Among the 43 tcrB-positive isolates, 35 were E. faecium and 8 were E. faecalis. The mean MICs of copper for tcrB-negative and tcrB-positive enterococci were 6.2 and 22.2 mM, respectively. The restriction digestion of the genomic DNA of E. faecium or E. faecalis with S1 nuclease yielded a band of ∼194-kbp size to which both tcrB and the erm(B) gene probes hybridized. A conjugation assay demonstrated cotransfer of tcrB and erm(B) genes between E. faecium and E. faecalis strains. The higher prevalence of tcrB-positive enterococci in piglets fed elevated copper compared to that in piglets fed normal copper suggests that supplementation of copper in swine diets selected for resistance.
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