ArticleJournal of experimental botany2011
Is the 6 kDa tobacco etch viral protein a bona fide ERES marker?
Article in Journal of experimental botany, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 39 citations in OpenAlex.
- Distinct Mechanisms of Endomembrane Reorganization Determine Dissimilar Transport Pathways in Plant RNA Viruses.Plants (Basel, Switzerland) · 2022Review
- Characterization ofFrontiers in plant science · 2022Article
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- p24 Family Proteins Are Involved in Transport to the Plasma Membrane of GPI-Anchored Proteins in Plants.Plant physiology · 2020Article
- Small hydrophobic viral proteins involved in intercellular movement of diverse plant virus genomes.AIMS microbiology · 2020Review
- Mutagenesis Scanning Uncovers Evolutionary Constraints on Tobacco Etch Potyvirus Membrane-Associated 6K2 Protein.Genome biology and evolution · 2019Article
- A Functional Link between RNA Replication and Virion Assembly in the PotyvirusJournal of virology · 2018Article
- The Cytokinin Oxidase/Dehydrogenase CKX1 Is a Membrane-Bound Protein Requiring Homooligomerization in the Endoplasmic Reticulum for Its Cellular Activity.Plant physiology · 2018Article
- Protein composition of 6K2-induced membrane structures formed during Potato virus A infection.Molecular plant pathology · 2016Article
- The Vesicle-Forming 6K2 Protein of Turnip Mosaic Virus Interacts with the COPII Coatomer Sec24a for Viral Systemic Infection.Journal of virology · 2015Article
- Traffic into silence: endomembranes and post-transcriptional RNA silencing.The EMBO journal · 2014Review
- Putative p24 complexes in Arabidopsis contain members of the delta and beta subfamilies and cycle in the early secretory pathway.Journal of experimental botany · 2013Article
- Host endomembrane recruitment for plant RNA virus replication.Current opinion in virology · 2012Review
- Impact on the endoplasmic reticulum and Golgi apparatus of turnip mosaic virus infection.Journal of virology · 2012Article
- Coupled transport of Arabidopsis p24 proteins at the ER-Golgi interface.Journal of experimental botany · 2012Article
- Arabidopsis senescence-associated protein DMP1 is involved in membrane remodeling of the ER and tonoplast.BMC plant biology · 2012Article
- Cytoplasmic Arabidopsis AGO7 accumulates in membrane-associated siRNA bodies and is required for ta-siRNA biogenesis.The EMBO journal · 2012Article
- ER Import Sites and Their Relationship to ER Exit Sites: A New Model for Bidirectional ER-Golgi Transport in Higher Plants.Frontiers in plant science · 2012Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The claim that the 6 kDa viral protein (VP) of Tobacco Etch Virus is a marker for ER exit sites (ERES) has been investigated. When transiently expressed as a CFP tagged fusion construct in tobacco mesophyll protoplasts, this integral membrane protein co-localizes with both the COPII coat protein YFP-SEC24 and the Golgi marker Man1-RFP. However, when over-expressed the VP locates to larger spherical structures which co-localize with neither ER nor Golgi markers. Nevertheless, deletion of the COPII interactive N-terminal D(X)E motif causes it to be broadly distributed throughout the ER, supporting the notion that this protein could be an ERES marker. Curiously, whereas brefeldin A (BFA) caused a typical Golgi-stack response (redistribution into the ER) of the VP in leaf epidermal cells, in protoplasts it resulted in the formation of structures identical to those formed by over-expression. However, anomalous results were obtained with protoplasts: when co-expressed with the non-cycling cis-Golgi marker Man1-RFP, a BFA-induced redistribution of the VP-CFP signal into the ER was observed, but, in the presence of the cycling Golgi marker ERD2-YFP, this did not occur. High resolution images of side-on views of Golgi stacks in epidermal cells showed that the 6 kDa VP-CFP signal overlapped considerably more with YFP-SEC24 than with Man1-RFP, indicating that the VP is proportionately more associated with ERES. However, based on a consideration of the structure of its cytoplasmic tail, the scenario that the VP collects at ERES and is transported to the cis-Golgi before being recycled back to the ER, is supported.
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