ArticleParasitology international2012
Evaluation of liver fluke recombinant cathepsin B-1 protease as a serodiagnostic antigen for human opisthorchiasis.
Article in Parasitology international, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
21 citing papers in PubMed, 42 citations in OpenAlex.
- Enhanced serodiagnosis of opisthorchiasis using a multi-epitope dot-ELISA: comparative evaluation of visual and imageJ-assisted analysis of IgG and IgM responses.BMC infectious diseases · 2026Article
- Multi-epitope protein production and its application in the diagnosis of opisthorchiasis.Parasites & vectors · 2024Article
- Diagnosis of Human Trematode Infections.Advances in experimental medicine and biology · 2024Review
- Exploring extracellular vesicles in zoonotic helminth biology: implications for diagnosis, therapeutic and delivery.Frontiers in cellular and infection microbiology · 2024Review
- Novel recombinant proteins and peptides fromBiochemistry and biophysics reports · 2023Article
- Production and Immunological Characterization of scFv Specific to Epitope ofTropical medicine and infectious disease · 2023Article
- Epidemiology and Control of Opisthorchis viverrini Infection: Implications for Cholangiocarcinoma Prevention.Recent results in cancer research. Fortschritte der Krebsforschung. Progres dans les recherches sur le cancer · 2023Article
- An Innovative Test for the Rapid Detection of Specific IgG Antibodies in Human Whole-Blood for the Diagnosis ofTropical medicine and infectious disease · 2022Article
- A first attempt at determining the antibody-specific pattern ofVeterinary world · 2022Article
- Comparative assessment of immunochromatographic test kits using somatic antigens from adult Opisthorchis viverrini and IgG and IgG4 conjugates for serodiagnosis of human opisthorchiasis.Parasitology research · 2021Article
- Development of an Immunochromatographic Point-of-Care Test for Serodiagnosis of Opisthorchiasis and Clonorchiasis.The American journal of tropical medicine and hygiene · 2019Article
- Immunomodulation by Helminths: Intracellular Pathways and Extracellular Vesicles.Frontiers in immunology · 2018Review
- Chicken IgY-based coproantigen capture ELISA for diagnosis of human opisthorchiasis.Parasitology international · 2017Article
- Enzyme-linked immunosorbent assay for diagnosis of Amphimerus spp. liver fluke infection in Humans.Memorias do Instituto Oswaldo Cruz · 2017Article
- Immunodiagnosis of opisthorchiasis using parasite cathepsin F.Parasitology research · 2015Article
- Systems biology studies of adult paragonimus lung flukes facilitate the identification of immunodominant parasite antigens.PLoS neglected tropical diseases · 2014Article
- Droplet electrospray ionization mass spectrometry for high throughput screening for enzyme inhibitors.Analytical chemistry · 2014Article
- Analysis of a novel cathepsin B circulating antigen and its response to drug treatment in Trichinella-infected mice.Parasitology research · 2013Article
- Expression of Neospora caninum NcSRS2 surface protein in Pichia pastoris and its application for serodiagnosis of Neospora infection.Pathogens and global health · 2013Article
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Corrections and comments
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
Abstract
A cathepsin B-like cysteine protease belonging to family C1 is abundantly expressed in the transcriptome and proteome of the carcinogenic liver fluke of humans, Opisthorchis viverrini. This enzyme is present in excretory/secretory (ES) products released by parasites cultured in vitro. This study evaluated the performance of recombinant O. viverrini cathepsin B1 (rOv-CB-1) as an antigen for immunodiagnosis of opisthorchiasis. The full length Ov-CB-1 cDNA was cloned and recombinant protein was produced in catalytically active form in Pichia pastoris. The recombinant Ov-CB-1 (rOv-CB-1) was affinity purified via nickel-NTA chromatography and tested in enzyme-linked immunosorbent assays (ELISA) with human sera from an opisthorchiasis endemic area. Sera from egg-positive O. viverrini infections produced a strong IgG antibody response to rOv-CB-1 both in ELISA and immunoblot analysis. The sensitivity and specificity of the ELISA test was 67% and 81%, respectively. These findings support the feasibility of using recombinant Ov-CB-1 in ELISA for the serodiagnosis of human opisthorchiasis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.