ArticleThe Journal of molecular diagnostics : JMD2011
RNA-stabilized whole blood samples but not peripheral blood mononuclear cells can be stored for prolonged time periods prior to transcriptome analysis.
Article in The Journal of molecular diagnostics : JMD, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Plasma-Free Blood as a Potential Alternative to Whole Blood for Transcriptomic Analysis.Phenomics (Cham, Switzerland) · 2024Article
- A single workflow for multi-species blood transcriptomics.BMC genomics · 2024Article
- Optimized protocol for the extraction of RNA and DNA from frozen whole blood sample stored in a single EDTA tube.Scientific reports · 2021Article
- RNA-sequencing analysis of differential gene expression associated with arterial stiffness.Vascular · 2020Article
- Blood co-expression modules identify potential modifier genes of diabetes and lung function in cystic fibrosis.PloS one · 2020Article
- Genome-Wide Patterns of Gene Expression in a Wild Primate Indicate Species-Specific Mechanisms Associated with Tolerance to Natural Simian Immunodeficiency Virus Infection.Genome biology and evolution · 2019Article
- Article
- Whole Blood mRNA Expression-Based Prognosis of Metastatic Renal Cell Carcinoma.International journal of molecular sciences · 2017Article
- Whole Blood RNA as a Source of Transcript-Based Nutrition- and Metabolic Health-Related Biomarkers.PloS one · 2016Article
- A cell-based systems biology assessment of human blood to monitor immune responses after influenza vaccination.PloS one · 2015Article
- Type I interferon signaling genes in recurrent major depression: increased expression detected by whole-blood RNA sequencing.Molecular psychiatry · 2014Article
- Biological and analytical stability of a peripheral blood gene expression score for obstructive coronary artery disease in the PREDICT and COMPASS studies.Journal of cardiovascular translational research · 2014Article
- Comparison of blood RNA extraction methods used for gene expression profiling in amyotrophic lateral sclerosis.PloS one · 2014Article
- Article
- Analysis of the transcriptome in molecular epidemiology studies.Environmental and molecular mutagenesis · 2013Review
- Epigenetic biomarkers: Current strategies and future challenges for their use in the clinical laboratory.Critical reviews in clinical laboratory sciencesReview
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7 authors.
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Abstract
Microarray-based transcriptome analysis of peripheral blood as surrogate tissue has become an important approach in clinical implementations. However, application of gene expression profiling in routine clinical settings requires careful consideration of the influence of sample handling and RNA isolation methods on gene expression profile outcome. We evaluated the effect of different sample preservation strategies (eg, cryopreservation of peripheral blood mononuclear cells or freezing of PAXgene-stabilized whole blood samples) on gene expression profiles. Expression profiles obtained from cryopreserved peripheral blood mononuclear cells differed substantially from those of their nonfrozen counterpart samples. Furthermore, expression profiles in cryopreserved peripheral blood mononuclear cell samples were found to undergo significant alterations with increasing storage period, whereas long-term freezing of PAXgene RNA stabilized whole blood samples did not significantly affect stability of gene expression profiles. This report describes important technical aspects contributing toward the establishment of robust and reliable guidance for gene expression studies using peripheral blood and provides a promising strategy for reliable implementation in routine handling for diagnostic purposes.
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