ArticleThe Journal of molecular diagnostics : JMD2011
Rapid, sensitive, and specific detection of Mycobacterium tuberculosis complex by real-time PCR on paraffin-embedded human tissues.
Article in The Journal of molecular diagnostics : JMD, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 2 of them syntheses that pooled it.
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Who cites it
19 citing papers in PubMed, 2 syntheses or guidelines pooled it, 54 citations in OpenAlex.
- Diagnostic accuracy of in-house real-time PCR assay for Mycobacterium tuberculosis: a systematic review and meta-analysis.BMC infectious diseases · 2019Pooled it
- Effectiveness of real-time polymerase chain reaction assay for the detection of Mycobacterium tuberculosis in pathological samples: a systematic review and meta-analysis.Systematic reviews · 2017Pooled it
- Prevalence and Genotypes of Mycobacteria Species in Formalin-Fixed Paraffin-Embedded Tissue Samples in a Tertiary Laboratory, Northern Pretoria, South Africa.Diagnostics (Basel, Switzerland) · 2026Article
- Detection and accurate identification of Mycobacterium species by flow injection tandem mass spectrometry (FIA-MS/MS) analysis of mycolic acids.Scientific reports · 2025Article
- Design and optimization of a multiplex real-time PCR assay for detection of Hepatitis C virus (HCV), Human immunodeficiency virus-1 (HIV-1) and Human hepegivirus-1 (HHpgV-1).Gastroenterology and hepatology from bed to bench · 2025Article
- Comorbidity of sarcoidosis and non-tuberculosis mycobacteria: a case description.Quantitative imaging in medicine and surgery · 2024Article
- Quantum Dot-Based Nanosensors for In Vitro Detection ofNanomaterials (Basel, Switzerland) · 2024Review
- Unveiling the Clinical Diversity in Nontuberculous Mycobacteria (NTM) Infections: A Comprehensive Review.Cureus · 2023Review
- Reactivated disseminated tuberculosis in pregnancy: Case report and review of the literature.Case reports in women's health · 2023Article
- Unbiased screen for pathogens in human paraffin-embedded tissue samples by whole genome sequencing and metagenomics.Frontiers in cellular and infection microbiology · 2022Article
- Performances of single tube nested polymerase chain reaction and GeneXpert ultra on Formalin fixed paraffin embedded tissues in the diagnosis of tuberculous spondylodiscitis.Clinical rheumatology · 2021Article
- [Histopathology of pulmonary tuberculosis].Der Pathologe · 2021Review
- Rapid Detection and Quantification ofFrontiers in microbiology · 2021Article
- Digital polymerase chain reaction for detecting c-MYC copy number gain in tissue and cell-free plasma samples of colorectal cancer patients.Scientific reports · 2019Article
- Does Polymerase Chain Reaction of Tissue Specimens Aid in the Diagnosis of Tuberculosis?Journal of pathology and translational medicine · 2016Article
- Comparison of characteristics of culture-negative pyogenic spondylitis and tuberculous spondylitis: a retrospective study.BMC infectious diseases · 2016Article
- Diagnosis of pulmonary tuberculosis: recent advances and diagnostic algorithms.Tuberculosis and respiratory diseases · 2015Review
- Article
- Effects of fixation and storage of human tissue samples on nucleic Acid preservation.Korean journal of pathology · 2014Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The detection of Mycobacterium tuberculosis complex (MTB) in clinical specimens is important for diagnosing and caring for patients in whom tuberculosis is clinically suspected. We collected 129 FFPE specimens, including 56 nontuberculosis cases, 63 MTB cases, and 10 nontuberculous mycobacteria (NTM) cases determined by acid-fast bacilli (AFB) culture. We performed AFB staining; nested MTB PCR, targeting the IS6110 gene; and real-time MTB PCR, targeting the senX3-regX3 intergenic region in the 129 FFPE specimens. The sensitivity and specificity of AFB staining were 37.0% and 98.2%, respectively, using AFB culture results as the reference standard. The sensitivity and specificity of detecting MTB were 68.3% and 98.5%, respectively, by nested PCR; and 74.6% and 98.5% by real-time PCR, respectively. Among the 129 specimens, four were positive by AFB staining but negative by nested or real-time PCR. NTM grew in all four of these cases by AFB culture. AFB density in FFPE tissue sections significantly correlated with MTB DNA load. Thus, real-time PCR is a useful diagnostic tool for rapid and sensitive MTB detection in FFPE specimens, whereas NTM should be included in differential diagnoses of cases positive by AFB staining but negative by PCR.
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