ArticleThe American journal of pathology2011
Two-photon imaging within the murine thorax without respiratory and cardiac motion artifact.
Article in The American journal of pathology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
48 citing papers in PubMed, 74 citations in OpenAlex.
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- Design and In Vivo Validation of a Constant-Flow Ventilation Circuit for Tissue Stabilization During Intravital Imaging of the Lungs.Annals of biomedical engineering · 2026Article
- Comparative Evaluation of Combined Denoising and Resolution Enhancement Algorithms for Intravital Two-Photon Imaging of Organs.Biosensors · 2025Article
- Stem cell migration drives lung repair in living mice.Developmental cell · 2024Article
- New Therapeutic Options in Pulmonal Diseases: Sphingolipids and Modulation of Sphingolipid Metabolism.Handbook of experimental pharmacology · 2024Review
- Does Chronic Obstructive Pulmonary Disease Originate from Different Cell Types?American journal of respiratory cell and molecular biology · 2023Article
- High dynamic range 3D motion tracking using circular scans with optical coherence tomography.Biomedical optics express · 2023Article
- A Permanent Window for Investigating Cancer Metastasis to the Lung.Journal of visualized experiments : JoVE · 2021Article
- The Indiana O'Brien Center for Advanced Renal Microscopic Analysis.American journal of physiology. Renal physiology · 2021Review
- Human umbilical cord mesenchymal stem cell-derived extracellular vesicles ameliorate airway inflammation in a rat model of chronic obstructive pulmonary disease (COPD).Stem cell research & therapy · 2021Article
- Digital Image Analysis Tools Developed by the Indiana O'Brien Center.Frontiers in physiology · 2021Review
- Actually Seeing What Is Going on - Intravital Microscopy in Tissue Engineering.Frontiers in bioengineering and biotechnology · 2021Review
- Intravital Imaging Techniques for Biomedical and Clinical Research.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2020Review
- Live imaging of the pulmonary immune environment.Cellular immunology · 2020Review
- Multicolor two-photon imaging of in vivo cellular pathophysiology upon influenza virus infection using the two-photon IMPRESS.Nature protocols · 2020Article
- Intravital Imaging of Pulmonary Immune Response in Inflammation and Infection.Frontiers in cell and developmental biology · 2020Review
- Quantitative Kinetic Models from Intravital Microscopy: A Case Study Using Hepatic Transport.The journal of physical chemistry. B · 2019Article
- Live Intravital Imaging of Cellular Trafficking in the Cardiac Microvasculature-Beating the Odds.Frontiers in immunology · 2019Review
- Bioactive Sphingolipids in the Pathogenesis of Chronic Obstructive Pulmonary Disease.Annals of the American Thoracic Society · 2018Review
- Review
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
Abstract
Intravital microscopy has been recognized for its ability to make physiological measurements at cellular and subcellular levels while maintaining the complex natural microenvironment. Two-photon microscopy (TPM), using longer wavelengths than single-photon excitation, has extended intravital imaging deeper into tissues, with minimal phototoxicity. However, due to a relatively slow acquisition rate, TPM is especially sensitive to motion artifact, which presents a challenge when imaging tissues subject to respiratory and cardiac movement. Thoracoabdominal organs that cannot be exteriorized or immobilized during TPM have generally required the use of isolated, pump-perfused preparations. However, this approach entails significant alteration of normal physiology, such as a lack of neural inputs, increased vascular resistance, and leukocyte activation. We adapted techniques of intravital microscopy that permitted TPM of organs maintained within the thoracoabdominal cavity of living, breathing rats or mice. We obtained extended intravital TPM imaging of the intact lung, arguably the organ most susceptible to both respiratory and cardiac motion. Intravital TPM detected the development of lung microvascular endothelial activation manifested as increased leukocyte adhesion and plasma extravasation in response to oxidative stress inducers PMA or soluble cigarette smoke extract. The pulmonary microvasculature and alveoli in the intact animal were imaged with comparable detail and fidelity to those in pump-perfused animals, opening the possibility for TPM of other thoracoabdominal organs under physiological and pathophysiological conditions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.