ArticleBMC microbiology2011
In vivo versus in vitro protein abundance analysis of Shigella dysenteriae type 1 reveals changes in the expression of proteins involved in virulence, stress and energy metabolism.
Article in BMC microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 51 citations in OpenAlex.
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- Global transcriptional regulator FNR regulates the pyruvate cycle and proton motive force to play a role in aminoglycosides resistance ofFrontiers in microbiology · 2022Article
- The identification of novel immunogenic antigens as potential Shigella vaccine components.Genome medicine · 2021Article
- Fnr Negatively Regulates Prodigiosin Synthesis inFrontiers in microbiology · 2021Article
- Plasma metabolites mediate the effect of HbA1c on incident cardiovascular disease.Clinical cardiology · 2019Article
- Identification of common highly expressed genes of Salmonella Enteritidis by in silico prediction of gene expression and in vitro transcriptomic analysis.Poultry science · 2019Article
- Protein aggregation in Ehrlichia chaffeensis during infection of mammalian cells.FEMS microbiology letters · 2017Article
- The genomic signatures of Shigella evolution, adaptation and geographical spread.Nature reviews. Microbiology · 2016Review
- Proteomic Analyses of Intracellular Salmonella enterica Serovar Typhimurium Reveal Extensive Bacterial Adaptations to Infected Host Epithelial Cells.Infection and immunity · 2015Article
- Mass spectrometry-based proteomic approaches to study pathogenic bacteria-host interactions.Protein & cell · 2015Review
- The genome of Shigella dysenteriae strain Sd1617 comparison to representative strains in evaluating pathogenesis.FEMS microbiology letters · 2015Article
- FNR regulates expression of important virulence factors contributing to pathogenicity of uropathogenic Escherichia coli.Infection and immunity · 2014Article
- NtrBC and Nac contribute to efficient Shigella flexneri intracellular replication.Journal of bacteriology · 2014Article
- Transcriptional regulation of bacterial virulence gene expression by molecular oxygen and nitric oxide.Virulence · 2014Review
- Identification of antigenic proteins of the nosocomial pathogen Klebsiella pneumoniae.PloS one · 2014Article
- Analysis of the proteome of intracellular Shigella flexneri reveals pathways important for intracellular growth.Infection and immunity · 2013Article
- Proteomic View of Interactions of Shiga Toxin-Producing Escherichia coli with the Intestinal Environment in Gnotobiotic Piglets.PloS one · 2013Article
- Characterizing the Escherichia coli O157:H7 proteome including protein associations with higher order assemblies.PloS one · 2011Article
- Repurposing p97 inhibitors for chemical modulation of the bacterial ClpB-DnaK bichaperone system.The Journal of biological chemistryArticle
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundShigella dysenteriae serotype 1 (SD1) causes the most severe form of epidemic bacillary dysentery. Quantitative proteome profiling of Shigella dysenteriae serotype 1 (SD1) in vitro (derived from LB cell cultures) and in vivo (derived from gnotobiotic piglets) was performed by 2D-LC-MS/MS and APEX, a label-free computationally modified spectral counting methodology.
resultsOverall, 1761 proteins were quantitated at a 5% FDR (false discovery rate), including 1480 and 1505 from in vitro and in vivo samples, respectively. Identification of 350 cytoplasmic membrane and outer membrane (OM) proteins (38% of in silico predicted SD1 membrane proteome) contributed to the most extensive survey of the Shigella membrane proteome reported so far. Differential protein abundance analysis using statistical tests revealed that SD1 cells switched to an anaerobic energy metabolism under in vivo conditions, resulting in an increase in fermentative, propanoate, butanoate and nitrate metabolism. Abundance increases of transcription activators FNR and Nar supported the notion of a switch from aerobic to anaerobic respiration in the host gut environment. High in vivo abundances of proteins involved in acid resistance (GadB, AdiA) and mixed acid fermentation (PflA/PflB) indicated bacterial survival responses to acid stress, while increased abundance of oxidative stress proteins (YfiD/YfiF/SodB) implied that defense mechanisms against oxygen radicals were mobilized. Proteins involved in peptidoglycan turnover (MurB) were increased, while β-barrel OM proteins (OmpA), OM lipoproteins (NlpD), chaperones involved in OM protein folding pathways (YraP, NlpB) and lipopolysaccharide biosynthesis (Imp) were decreased, suggesting unexpected modulations of the outer membrane/peptidoglycan layers in vivo. Several virulence proteins of the Mxi-Spa type III secretion system and invasion plasmid antigens (Ipa proteins) required for invasion of colonic epithelial cells, and release of bacteria into the host cell cytosol were increased in vivo.
conclusionsGlobal proteomic profiling of SD1 comparing in vivo vs. in vitro proteomes revealed differential expression of proteins geared towards survival of the pathogen in the host gut environment, including increased abundance of proteins involved in anaerobic energy respiration, acid resistance and virulence. The immunogenic OspC2, OspC3 and IpgA virulence proteins were detected solely under in vivo conditions, lending credence to their candidacy as potential vaccine targets.
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