ArticleMethods in molecular biology (Clifton, N.J.)2011
Use of colloidal silica-beads for the isolation of cell-surface proteins for mass spectrometry-based proteomics.
Article in Methods in molecular biology (Clifton, N.J.), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed.
- Targeting splicing-derived neoantigens for precision cancer immunotherapy.Frontiers in immunology · 2026Review
- Organelle resolved proteomics uncovers PLA2R1 as a novel cell surface marker required for chordoma growth.Acta neuropathologica communications · 2024Article
- Comprehensive Discovery of the Accessible Primary Amino Group-Containing Segments from Cell Surface Proteins by Fine-Tuning a High-Throughput Biotinylation Method.International journal of molecular sciences · 2022Article
- Facile Preparation of Peptides for Mass Spectrometry Analysis in Bottom-Up Proteomics Workflows.Current protocols · 2021Article
- CIRFESS: An Interactive Resource for Querying the Set of Theoretically Detectable Peptides for Cell Surface and Extracellular Enrichment Proteomic Studies.Journal of the American Society for Mass Spectrometry · 2020Article
- Review
- Designer nanoparticle: nanobiotechnology tool for cell biology.Nano convergence · 2016Review
- Analysis of lipid-composition changes in plasma membrane microdomains.Journal of lipid research · 2015Article
- Coupling enrichment methods with proteomics for understanding and treating disease.Proteomics. Clinical applications · 2015Review
- A mass spectrometric-derived cell surface protein atlas.PloS one · 2015Article
- Cell surface profiling using high-throughput flow cytometry: a platform for biomarker discovery and analysis of cellular heterogeneity.PloS one · 2014Article
- The pancreatic beta cell surface proteome.Diabetologia · 2012Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Chaney and Jacobson first introduced the colloidal silica-bead protocol for the coating of cellular plasma membranes in the early 1980s. Since then, this method has been successfully incorporated into a wide range of in vitro and in vivo applications for the isolation of cell-surface proteins. The principle is simple - cationic colloidal silica microbeads are introduced to a suspension or monolayer of cells in culture. Electrostatic interactions between the beads and the negatively charged plasma membrane, followed by cross-linking to the membrane with an anionic polymer, ensure attachment and maintain the native protein conformation. Cells are subsequently ruptured, and segregation of the resulting plasma membrane sheets from the remaining- cell constituents is achieved by ultracentrifugation through density gradients. The resulting membrane-bead pellet is treated with various detergents or chaotropic agents (i.e., urea) to elute bound proteins. If proteomic profiling by mass spectrometry is desired, proteins are denatured, carbamidomethylated, and digested into peptides prior to chromatography.
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Registered trials
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