ArticleTransgenic research2012
Engineering broad-spectrum resistance against RNA viruses in potato.
Article in Transgenic research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 59 citations in OpenAlex.
- MicroRNA-Induced Gene Silencing (MIGS): A Tool for Multi-Gene Silencing and Targeting Viruses in Plants.Plant biotechnology journal · 2026Article
- Stable Resistance to Potato Virus Y and Potato Leafroll Virus in Transgenic Potato Plants cv. Kennebec Expressing Viral Genes Under Greenhouse and Field Conditions.Plants (Basel, Switzerland) · 2026Article
- Article
- RNA silencing: the future potential strategy for engineering virus resistance in plants.Physiology and molecular biology of plants : an international journal of functional plant biology · 2025Review
- Article
- Genome editing (CRISPR-Cas)-mediated virus resistance in potato (Solanum tuberosum L.).Molecular biology reports · 2022Review
- Comparative evaluation of resistance to potato virus Y (PVY) in three different RNAi-based transgenic potato plants.Transgenic research · 2022Article
- Review
- Small RNA-based plant protection against diseases.Frontiers in plant science · 2022Review
- RNA interference and crop protection against biotic stresses.Physiology and molecular biology of plants : an international journal of functional plant biology · 2021Review
- Prospects for potato genome editing to engineer resistance against viruses and cold-induced sweetening.GM crops & food · 2020Review
- Bacterium-Mediated RNA Interference: Potential Application in Plant Protection.Plants (Basel, Switzerland) · 2019Review
- Mechanisms of Plant Tolerance to RNA Viruses Induced by Plant-Growth-Promoting Microorganisms.Plants (Basel, Switzerland) · 2019Review
- Review
- Utilization of engineered resistance to viruses in crops of the developing world, with emphasis on sub-Saharan Africa.Current opinion in virology · 2017Review
- RNAi-Mediated Simultaneous Resistance Against Three RNA Viruses in Potato.Molecular biotechnology · 2017Article
- Small RNA Based Genetic Engineering for Plant Viral Resistance: Application in Crop Protection.Frontiers in microbiology · 2017Review
- Marker-free PLRV resistant potato mediated by Cre-loxP excision and RNAi.Transgenic research · 2016Article
- Engineered resistance in potato against potato leafroll virus, potato virus A and potato virus Y.Virus genes · 2013Article
- Overexpression of the wild potato eIF4E-1 variant Eva1 elicits Potato virus Y resistance in plants silenced for native eIF4E-1.Transgenic research · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA silencing technology has become the tool of choice for inducing resistance against viruses in plants. A significant discovery of this technology is that double-stranded RNA (dsRNA), which is diced into small interfering RNAs (siRNAs), is a potent trigger for RNA silencing. By exploiting this phenomenon in transgenic plants, it is possible to confer high level of virus resistance by specific targeting of cognate viral RNA. In order to maximize the efficiency and versatility of the vector-based siRNA approach, we have constructed a chimeric expression vector containing three partial gene sequences derived from the ORF2 gene of Potato virus X, Helper Component Protease gene of Potato virus Y and Coat protein gene of Potato leaf roll virus. Solanum tuberosum cv. Desiree and Kuroda were transformed with this chimeric gene cassette via Agrobacterium tumefaciens-mediated transformation and transgenic status was confirmed by PCR, Southern and double antibody sandwich ELISA detection. Due to simultaneous RNA silencing, as demonstrated by accumulation of specific siRNAs, the expression of partial triple-gene sequence cassette depicted 20% of the transgenic plants are immune against all three viruses. Thus, expression of a single transgene construct can effectively confer resistance to multiple viruses in transgenic plants.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.